Unique gene expression profile of the proliferating Xenopus tadpole tail blastema cells deciphered by RNA-sequencing analysis

PloS One
Hiroshi TsujiokaTakeo Kubo

Abstract

Organ regenerative ability depends on the animal species and the developmental stage. The molecular bases for variable organ regenerative ability, however, remain unknown. Previous studies have identified genes preferentially expressed in the blastema tissues in various animals, but transcriptome analysis of the isolated proliferating blastema cells has not yet been reported. In the present study, we used RNA-sequencing analysis to analyze the gene expression profile of isolated proliferating blastema cells of regenerating Xenopus laevis tadpole tails. We used flow cytometry to isolate proliferating cells, and non-proliferating blastema cells, from regenerating tadpole tails as well as proliferating tail bud cells from tail bud embryos, the latter two of which were used as control cells, based on their DNA content. Among the 28 candidate genes identified by RNA-sequencing analysis, quantitative reverse transcription-polymerase chain reaction identified 10 genes whose expression was enriched in regenerating tadpole tails compared with non-regenerating tadpole tails or tails from the tail bud embryos. Among them, whole mount in situ hybridization revealed that chromosome segregation 1-like and interleukin 11 were expressed in the...Continue Reading

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Citations

Feb 20, 2016·Developmental and Comparative Immunology·Anthony L MescherMichael W King
Sep 17, 2020·Proceedings. Biological Sciences·Kellen Matos VerissimoIgor Schneider
Feb 26, 2019·Frontiers in Physiology·Anneke D Kakebeen, Andrea E Wills
Oct 18, 2018·Nature Communications·Fernando FerreiraMin Zhao
Nov 2, 2018·International Journal of Molecular Sciences·Sudip PaudelMargaret Saha
Nov 3, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Anneke Dixie KakebeenAndrea Elizabeth Wills

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Datasets Mentioned

BETA
AB933565

Methods Mentioned

BETA
amputation
flow cytometry
reverse transcription-PCR

Software Mentioned

blast
TopHat
Cuffdiff
Cufflinks
Xenbase

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