Unique gene expression profiles of heart allograft rejection in the interferon regulatory factor-1-deficient mouse

Transplant Immunology
Laurie EricksonHongsi Jiang

Abstract

Interferon regulatory factor-1 (IRF1) is a transcription factor for many genes involved in innate and adaptive immune responses. By using DNA array technology, we have previously demonstrated that IRF1 is significantly upregulated during acute rejection in rat heart allografts and is restored to isograft levels when recipients are treated with the immunosuppressants tacrolimus or cyclosporin A (CsA). To understand the precise role of IRF1 in transplant rejection, we investigated the rejection responses of mice completely deficient of IRF1 protein. Heterotopic heart transplantations were performed using C57BL/6J wild-type (WT B6) and IRF1-deficient (IRF1-/-) mice as recipients, and C3H mice as donors. Graft survival was determined by abdominal palpation and rejection was confirmed by histology. On day 6 after transplantation, isografts and allografts were harvested and subjected to gene expression analysis by a commercial nylon array and by real-time RT-PCR. Median survival time of heart allografts was 8 days in the WT B6 mice and 10 days in the IRF1-/- mice. The gene expression profiles of allografts from the WT B6 and IRF1-/- recipients were nearly identical to each other and very different from the profile of the isograft con...Continue Reading

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Citations

Jan 5, 2007·Proceedings of the American Thoracic Society·Jeffrey D LandeMarshall I Hertz
Nov 26, 2010·The Journal of Biological Chemistry·Xuesong QianJianguo Liu
Oct 14, 2011·Journal of Translational Medicine·Tara L SpiveyDavide Bedognetti
Apr 29, 2009·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Naoki HamaMorito Monden

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