Unique glycine-activated riboswitch linked to glycine-serine auxotrophy in SAR11.

Environmental Microbiology
H J TrippStephen J Giovannoni

Abstract

The genome sequence of the marine bacterium 'Candidatus Pelagibacter ubique' and subsequent analyses have shown that while it has a genome as small as many obligate parasites, it nonetheless possesses a metabolic repertoire that allows it to grow as one of the most successful free-living cells in the ocean. An early report based on metabolic reconstruction indicated that SAR11 cells are prototrophs for all amino acids. However, here we report experimental evidence that 'Cand. P. ubique' is effectively auxotrophic for glycine and serine. With glucose and acetate added to seawater to supply organic carbon, the addition of 125 nM to 1.5 microM glycine to growth medium containing all other nutrients in excess resulted in a linear increase in maximum cell density from 1.14 x 10(6) cells ml(-1) to 8.16 x 10(6) cells ml(-1) (R(2) = 0.992). Serine was capable of substituting for glycine at 1.5 microM. 'Cand. P. ubique' contains a glycine-activated riboswitch preceding malate synthase, an unusual genomic context that is conserved in the SAR11 group. Malate synthase plays a critical role in central metabolism by enabling TCA intermediates to be regenerated through the glyoxylate cycle. In vitro analysis of this riboswitch indicated that ...Continue Reading

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Citations

Apr 30, 2013·The Journal of Microbiology·H James Tripp
Aug 31, 2012·The ISME Journal·Scott M GiffordMary Ann Moran
Aug 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Stephen J Giovannoni
Jun 1, 2010·Journal of Bacteriology·Kirsten F BlockRonald R Breaker
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Karsten Zengler
Sep 3, 2011·PloS One·Jing SunStephen J Giovannoni
Feb 1, 2012·PloS One·Naiara Rodríguez-Ezpeleta, T Martin Embley
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ian LidburyYin Chen
Apr 18, 2014·The ISME Journal·Stephen J GiovannoniBen Temperton
Jul 22, 2009·Biochimica Et Biophysica Acta·Alexander Serganov, Dinshaw J Patel
Mar 3, 2009·Current Opinion in Microbiology·Michael D Dambach, Wade C Winkler
Mar 13, 2014·Environmental Microbiology·Sara BeierIngrid Obernosterer
Mar 4, 2014·Biochimica Et Biophysica Acta·Eric I Sun, Dmitry A Rodionov
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Aug 20, 2015·Environmental Microbiology·Sylvie EstrelaBenjamin Kerr
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