Unique modifications with phosphocholine and phosphoethanolamine define alternate antigenic forms of Neisseria gonorrhoeae type IV pili

Proceedings of the National Academy of Sciences of the United States of America
F T HeggeMichael Koomey

Abstract

Several major bacterial pathogens and related commensal species colonizing the human mucosa express phosphocholine (PC) at their cell surfaces. PC appears to impact host-microbe biology by serving as a ligand for both C-reactive protein and the receptor for platelet-activating factor. Type IV pili of Neisseria gonorrhoeae (Ng) and Neisseria meningitidis, filamentous protein structures critical to the colonization of their human hosts, are known to react variably with monoclonal antibodies recognizing a PC epitope. However, the structural basis for this reactivity has remained elusive. To address this matter, we exploited the finding that the PilE pilin subunit in Ng mutants lacking the PilV protein acquired the PC epitope independent of changes in pilin primary structure. Specifically, we show by using mass spectrometry that PilE derived from the pilV background is composed of a mixture of subunits bearing O-linked forms of either phosphoethanolamine (PE) or PC at the same residue, whereas the wild-type background carries only PE at that same site. Therefore, PilV can influence pilin structure and antigenicity by modulating the incorporation of these alternative modifications. The disaccharide covalently linked to Ng pilin was ...Continue Reading

References

Apr 1, 1989·Clinical Microbiology Reviews·J E Heckels
May 1, 1987·The Journal of Experimental Medicine·J SwansonJ M Koomey
May 1, 1995·Molecular Microbiology·N E FreitagM Koomey
Jan 1, 1996·Advances in Experimental Medicine and Biology·D R CundellN P Gerard
Aug 1, 1997·Biochemical Society Transactions·A K MenonJ Vidugiriene
Oct 10, 1998·Molecular Microbiology·M P JenningsE R Moxon
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M WolfgangM Koomey
Apr 28, 2000·Microbiology·P M PowerM P Jennings
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·H C Winther-LarsenM Koomey
Jul 18, 2002·The Journal of Experimental Medicine·Asesh BanerjeePeter A Rice
Aug 21, 2002·The Journal of Biological Chemistry·N Martin YoungChristine M Szymanski

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Citations

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Jul 5, 2012·The Journal of Biological Chemistry·Nichollas E ScottStuart J Cordwell
Aug 1, 2008·The Journal of Biological Chemistry·Nelson B Olivier, Barbara Imperiali
Nov 28, 2012·Infection and Immunity·Thomas W CullenM Stephen Trent
Dec 12, 2012·Infection and Immunity·Sarah E Clark, Jeffrey N Weiser
Aug 2, 2011·Journal of Bacteriology·Rebecca M ThomasJoann L Prior
Sep 25, 2007·Journal of Bacteriology·Amirreza FaridmoayerMario F Feldman
Jan 11, 2011·Journal of Bacteriology·Kai-Malte BartelsFrank Rosenau
Dec 4, 2012·Microbiology and Molecular Biology Reviews : MMBR·Carmen L GiltnerLori L Burrows
Jan 1, 2010·International Journal of Microbiology·Anne DellPaul Hitchen
Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Sandy Y M NgKen F Jarrell
Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Johanna K Hansen, Katrina T Forest
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Nov 30, 2011·The Journal of Cell Biology·Eric AlixCraig R Roy
Sep 25, 2015·ELife·Enno R OldewurtelBerenike Maier
Aug 11, 2005·Molecular Microbiology·Lori L Burrows
Jul 15, 2005·Annals of the New York Academy of Sciences·Chih-Te WuPaul A Davis
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