Unique molecular signatures of microRNAs in ocular fluids and plasma in diabetic retinopathy

PloS One
Zeljka Smit-McBrideLawrence S Morse

Abstract

The main objective of this pilot study was to identify circulatory microRNAs in aqueous or plasma that were reflecting changes in vitreous of diabetic retinopathy patients. Aqueous, vitreous and plasma samples were collected from a total of 27 patients undergoing vitreoretinal surgery: 11 controls (macular pucker or macular hole patients) and 16 with diabetes mellitus(DM): DM-Type I with proliferative diabetic retinopathy(PDR) (DMI-PDR), DM Type II with PDR(DMII-PDR) and DM Type II with nonproliferative DR(DMII-NPDR). MicroRNAs were isolated using Qiagen microRNeasy kit, quantified on BioAnalyzer, and profiled on Affymetrix GeneChip miRNA 3.0 microarrays. Data were analyzed using Expression Console, Transcriptome Analysis Console, and Ingenuity Pathway Analysis. The comparison analysis of circulatory microRNAs showed that out of a total of 847 human microRNA probes on the microarrays, common microRNAs present both in aqueous and vitreous were identified, and a large number of unique microRNA, dependent on the DM type and severity of retinopathy. Most of the dysregulated microRNAs in aqueous and vitreous of DM patients were upregulated, while in plasma, they were downregulated. Dysregulation of miRNAs in aqueous did not appear t...Continue Reading

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Citations

Jul 28, 2021·International Ophthalmology·Hui WangQian Ren

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Datasets Mentioned

BETA
GSE140959

Methods Mentioned

BETA
Chips
PCR
RNA seq
RNA-seq

Software Mentioned

MiRNA Target Filter
Affymetrix
IPA
Transcriptome Analysis Console ( TAC
Expression Console
Transcriptome Analysis Console
Ingenuity Pathway Analysis ( IPA

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