Abstract
A panel of monoclonal antibodies, designated HMB 18, 45, and 50, have been isolated that are highly specific for malignant melanoma. When tested on fixed, paraffin-embedded tissue sections, they reacted with 97 percent of melanomas tested (58 of 60), including pigmented, unpigmented, primary, and metastatic melanoma. The specificity in differentiating melanomas from other malignant tumors, including 112 carcinomas, 35 lymphomas, and 39 sarcomas, was 100 percent. Normal melanocytes were unreactive, although some benign melanocytic lesions were recognized. Using immunoprecipitation and SDS-PAGE analysis of 35S-methionine-labeled melanoma cells in tissue culture, a previously undescribed protein of approximately 10 kd was recognized by all three antibodies. HMB 50 also precipitated two high molecular weight proteins of 97 kd and 110 kd from the conditioned medium of melanoma cells. These monoclonal antibodies are the most sensitive and specific antibodies generated against human melanoma to date. Their clinical application in diagnostic surgical pathology and potential use in immunotherapy are discussed.
References
Feb 1, 1979·Virology·R C NowinskiW N Burnette
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1979·Annals of Surgery·D P KapelanskiM Kaufman
Jul 1, 1976·European Journal of Immunology·G Köhler, C Milstein
Jan 1, 1976·International Journal of Dermatology·A J Sober
Aug 7, 1975·Nature·G Köhler, C Milstein
Jan 29, 1976·The New England Journal of Medicine·F R EilberK P Ramming
Feb 15, 1985·Cancer·J M SterchiM R Cooper
Aug 15, 1970·Nature·U K Laemmli
Nov 1, 1970·Annals of Surgery·A Breslow
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J P BrownK E Hellström
Nov 1, 1982·The Journal of Cell Biology·A M Gown, A M Vogel
Dec 1, 1983·The Journal of Clinical Investigation·S M LarsonK E Hellström
Apr 1, 1981·Analytical Biochemistry·W N Burnette
Dec 1, 1982·The American Journal of Surgical Pathology·T NakajimaY Shimosato
Apr 1, 1984·Journal of Surgical Oncology·L J HumphreyF J Volenec
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R G WoodburyK E Hellström
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W G DippoldL J Old
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M Eisinger, O Marko
Apr 1, 1982·The Journal of Experimental Medicine·C S PukelL J Old
Mar 11, 1982·Nature·J P BrownW J Dreyer
Citations
Jan 1, 1989·Anatomy and Embryology·K A HolbrookH Kimball
Jan 1, 1990·Virchows Archiv. A, Pathological Anatomy and Histopathology·P LoddingL Angervall
Dec 1, 1995·Journal of the American Academy of Dermatology·L M Cohen
Jan 1, 1992·Journal of Dermatological Science·M IshiharaR J Zarbo
Aug 10, 2002·Seminars in Oncology·John A Thompson
Mar 17, 2010·The Journal of Biological Chemistry·Ralf M LeonhardtPeter Cresswell
Jan 21, 2011·The Journal of Biological Chemistry·Ralf M LeonhardtPeter Cresswell
Nov 10, 2007·The Journal of Biological Chemistry·Dawn C HarperMichael S Marks
Feb 1, 1994·DNA and Cell Biology·G A MareshH Marquardt
Nov 6, 2001·Molecular Biology of the Cell·J F BersonM S Marks
Jun 9, 2006·Molecular Biology of the Cell·Alexander C TheosMichael S Marks
Sep 1, 1996·The British Journal of Dermatology·A KikuchiT Nishikawa
Jan 1, 1991·Cytopathology : Official Journal of the British Society for Clinical Cytology·K NasiellL Skoog
Dec 1, 1991·Journal of Cutaneous Pathology·H Kamino, S T Tam
Oct 1, 1991·Journal of Cutaneous Pathology·B R SmollerJ Krueger
Sep 1, 1995·Journal of Clinical Pathology·E Cohen-KnafoG Delsol
Aug 26, 1999·American Journal of Respiratory Cell and Molecular Biology·Y MatsumotoJ Moss
May 23, 2012·PloS One·Mei YuKevin J McElwee
Jan 29, 2013·Pigment Cell & Melanoma Research·Brenda WattMichael S Marks
Nov 1, 1992·Experimental Eye Research·R Y Kim, G J Wistow
Feb 1, 1996·Journal of the American Academy of Dermatology·M L Wallace, B R Smoller
Jan 4, 2007·The British Journal of Dermatology·S AmbrettiM Musiani
Jan 1, 1989·Human Pathology·A PodestaP H Duray
Mar 1, 1989·Human Pathology·P L FitzgibbonsS E Martin
Nov 24, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Xiaoning Zhe, Lucia Schuger
Dec 6, 2000·Veterinary Pathology·J A Ramos-VaraS J Kottler
Sep 6, 2001·Diagnostic Cytopathology·D Elliott, M B Pitman
Jan 31, 1998·The American Journal of Dermatopathology·H G SkeltonJ H Graham
Jan 26, 2020·The Journal of Investigative Dermatology·Silvia Benito-MartínezCédric Delevoye