Unique structural characteristics of the rabbit prion protein.

The Journal of Biological Chemistry
Yi WenDonghai Lin

Abstract

Rabbits are one of the few mammalian species that appear to be resistant to transmissible spongiform encephalopathies due to the structural characteristics of the rabbit prion protein (RaPrP(C)) itself. Here, we determined the solution structures of the recombinant protein RaPrP(C)-(91-228) and its S173N variant and detected the backbone dynamics of their structured C-terminal domains-(121-228). In contrast to many other mammalian PrP(C)s, loop 165-172, which connects β-sheet-2 and α-helix-2, is well-defined in RaPrP(C). For the first time, order parameters S(2) are obtained for residues in this loop region, indicating that loop 165-172 of RaPrP(C) is highly ordered. Compared with the wild-type RaPrP(C), less hydrogen bonds form in the S173N variant. The NMR dynamics analysis reveals a distinct increase in the structural flexibility of loop 165-172 and helix-3 after the S173N substitution, implying that the S173N substitution disturbs the long range interaction of loop 165-172 with helix-3, which further leads to a marked decrease in the global conformational stability. Significantly, RaPrP(C) possesses a unique charge distribution, carrying a continuous area of positive charges on the surface, which is distinguished from other...Continue Reading

References

Apr 5, 1992·Journal of Molecular Biology·P L Privalov, G I Makhatadze
Jan 1, 1991·Annual Review of Biochemistry·K A Dill, D Shortle
Jun 18, 1991·Biochemistry·D O Alonso, K A Dill
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·K KanekoS B Prusiner
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M PanF E Cohen
Aug 11, 1994·Nature·D A KociskoB Caughey
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Sep 1, 1995·Journal of Biomolecular NMR·N A FarrowL E Kay
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Jan 1, 1996·Annual Review of Physical Chemistry·K T DayieJ F Lefèvre
Dec 1, 1996·Journal of Biomolecular NMR·R A LaskowskiJ M Thornton
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·M BilleterK Wüthrich
Jul 25, 1997·Journal of Molecular Biology·K KanekoS B Prusiner
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·K KanekoS B Prusiner
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·T L JamesF E Cohen
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·D G DonneH J Dyson
Jul 17, 1998·Nature Structural Biology·L E Kay
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·S B Prusiner
Dec 5, 1998·The Journal of Biological Chemistry·P C Pauly, D A Harris
Nov 5, 1999·The Biochemical Journal·D R BrownI M Jones
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·R ZahnK Wüthrich
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·F López GarciaK Wüthrich
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·L CalzolaiK Wüthrich
Nov 7, 2002·Advances in Protein Chemistry·H Jane Dyson, Peter E Wright
May 27, 2003·Journal of Biomolecular NMR·Roger Cole, J Patrick Loria
Jul 16, 2003·Journal of Neurochemistry·Neville Vassallo, Jochen Herms
Sep 4, 2003·The Journal of Biological Chemistry·Jan ZieglerStephan Schwarzinger

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Citations

Jan 10, 2014·Journal of the American Chemical Society·Romany N N AbskharonJan Steyaert
Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Fred F DambergerKurt Wüthrich
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Barbara ChristenKurt Wüthrich
Sep 8, 2010·The Journal of Biological Chemistry·Pedro Fernandez-FunezDiego E Rincon-Limas
Aug 31, 2011·Journal of Biomolecular Structure & Dynamics·Jiapu Zhang
Apr 8, 2011·Journal of Biomolecular Structure & Dynamics·Jiapu Zhang, David D W Liu
Sep 1, 2012·Journal of Biomolecular Structure & Dynamics·Xiaojing CongGiuseppe Legname
Nov 5, 2013·Journal of Theoretical Biology·Jiapu Zhang, Yuanli Zhang
Oct 11, 2011·Cellular and Molecular Life Sciences : CMLS·Rafael LindenLuis Mauricio T R Lima
Aug 24, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabriele GiachinGiuseppe Legname
Jun 24, 2015·Journal of Biomolecular Structure & Dynamics·Jiapu Zhang, Feng Wang
Sep 10, 2015·Acta Biochimica Et Biophysica Sinica·Kejiang LinDonghai Lin
Sep 25, 2015·Journal of Biomolecular NMR·Ziming ZhangIoannis Gelis
Aug 28, 2012·Journal of Molecular Biology·Barbara ChristenKurt Wüthrich
Jun 6, 2015·Journal of Biomolecular Structure & Dynamics·Jiapu ZhangSubhojyoti Chatterjee
Sep 8, 2012·Prion·Natalia Fernández-BorgesJoaquín Castilla
Oct 12, 2010·Biochimica Et Biophysica Acta·Edward Málaga-TrilloTheodoros Sklaviadis
Sep 29, 2011·Biophysical Journal·Zheng ZhouYi Liang
Mar 13, 2014·Biophysical Journal·Wei ChenValerie Daggett
Aug 17, 2016·PloS One·Ziyao YuDonghai Lin
Oct 30, 2016·Acta Biochimica Et Biophysica Sinica·Yuanhui YuDonghai Lin
Nov 12, 2016·Biochemical and Biophysical Research Communications·Valerija KovačVladka Čurin Šerbec
Apr 9, 2013·Acta Biochimica Et Biophysica Sinica·Jiapu Zhang, Yuanli Zhang
Sep 18, 2013·Acta Biochimica Et Biophysica Sinica·Zhen YuanLifeng Yang
Apr 6, 2013·Acta Biochimica Et Biophysica Sinica·Pei HuangDonghai Lin
Sep 22, 2017·The Journal of Biological Chemistry·Timothy D KurtChristina J Sigurdson
Nov 14, 2017·PLoS Pathogens·Natalia Fernández-BorgesJoaquín Castilla
Oct 8, 2011·Communicative & Integrative Biology·Pedro Fernandez-FunezDiego E Rincon-Limas
Nov 3, 2016·The Biochemical Journal·Ankit SrivastavaBishwajit Kundu
Aug 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Enrico CaldaruloMichele Parrinello
Apr 15, 2017·Scientific Reports·Anatoly MalevanetsShoshana J Wodak

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