Unique tryptic peptides specific for bovine and human hemoglobin in the detection and confirmation of hemoglobin-based oxygen carriers

Analytical Chemistry
Fuyu GuanBernd Driessen

Abstract

Hemoglobin-based oxygen carriers (HBOCs) of bovine hemoglobin (Hb) or human Hb origin were developed for replacement or augmentation of blood during transfusion and have the potential to increase oxygen-carrying capacity of circulating blood and thus improve tissue oxygen delivery. Due to their potential for increasing oxygen-carrying capacity of circulating blood, they are excellent candidates for abuse in human and equine athletes. To deter athletes from blood doping with HBOCs such as Hemopure and Oxyglobin (OXY), a method for detection, confirmation, quantification, and distinguishing of HBOCs from native hemoglobin in test samples is needed. The purpose of this study was to identify unique peptides specific for bovine Hb and human Hb that are useful in the detection and confirmation of HBOCs in test samples. The LC-MS chromatographic peak profiles of tryptic digests from OXY, bovine Hb, human Hb, and equine Hb were compared, and unique tryptic peptides specific for bovine Hb, human Hb, and equine Hb were identified. The peptides specific for bovine Hb and OXY are related to bovine Hb alpha chain residues 69-90 and beta chain residues 40-58. The peptides specific for human Hb are related to human Hb alpha chain residues 63-...Continue Reading

References

Nov 1, 1984·Biomedical Mass Spectrometry·P Roepstorff, J Fohlman
Mar 29, 1996·The Journal of Biological Chemistry·M MartaB Giardina
Mar 12, 1999·Annual Review of Medicine·R M Winslow
Jun 1, 2000·The New England Journal of Medicine·H G Klein
Nov 24, 2001·International Journal of Sports Medicine·Y O SchumacherH Northoff

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Citations

May 1, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lidia Gebicka, Ewa Banasiak
May 28, 2005·Anesthesia and Analgesia·Lawrence R SomaBernd Driessen

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