Universal assay of vitellogenin as a biomarker for environmental estrogens

Environmental Health Perspectives
S A HeppellC V Sullivan

Abstract

Vitellogenin (VTG), the serum phospholipoglycoprotein precursor to egg yolk, is potentially an ideal biomarker for environmental estrogens. This study was undertaken to develop antibodies against conserved regions on the VTG molecule that could form the basis for establishing bioassays to detect estrogen exposure in any oviparous vertebrate. We developed monoclonal antibodies (mAbs) generated against purified rainbow trout (Oncorhynchus mykiss) VTG and selected for the property of specifically recognizing VTG purified from two phylogenetically distant vertebrates, trout and striped bass (Morone saxatilis). Results of enzyme-linked immunosorbent assay and Western blotting indicated that these mAbs specifically recognize purified VTG and VTG or other estrogen-inducible proteins in plasma or serum from representative species of four vertebrate classes (fish, amphibians, reptiles, and birds). All of the mAbs generated were IgM class. A polyclonal antiserum was raised against a synthetic consensus peptide representing the conserved N-terminal amino acid sequence of VTG. The results of Western blotting indicate that this antiserum specifically recognizes VTG in plasma or serum from teleost fish of diverse families. It was used to det...Continue Reading

References

Sep 1, 1991·General and Comparative Endocrinology·C PelisseroS Kaushick
Aug 6, 1990·Biochimica Et Biophysica Acta·S StifaniW J Schneider
Sep 1, 1990·International Journal of Peptide and Protein Research·D S KingG B Fields
Jan 1, 1989·Progress in Biophysics and Molecular Biology·B M ByrneG Ab
Aug 1, 1988·Trends in Genetics : TIG·W Wahli
Dec 15, 1988·The Biochemical Journal·M E Baker
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M CovensA De Loof
Jan 1, 1985·Developmental Biology·R A Wallace
Dec 7, 1984·Journal of Chromatography·B A BidlingmeyerT L Tarvin
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Apr 17, 1981·Science·W WahliR Weber
Sep 1, 1993·Pediatric Dermatology·P R CohenK Adler-Storthz
Mar 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·C PelisseroJ P Sumpter
Oct 1, 1995·Environmental Health Perspectives·J P Sumpter, S Jobling
Jul 1, 1993·Fish Physiology and Biochemistry·Y TaoC V Sullivan

❮ Previous
Next ❯

Citations

May 23, 2014·Aquatic Toxicology·Stéphanie BourrachotChristelle Adam-Guillermin
Feb 1, 1997·Environmental Health Perspectives·G A LeBlanc, L J Bain
Apr 16, 1998·Environmental Health Perspectives·T M CrispY M Patel
Jan 8, 2003·Environmental Health Perspectives·Lesley J MillsGerald E Zaroogian
Apr 5, 2003·Environmental Health Perspectives·Shannon AtkinsonAnn M Tarrant
Jul 5, 2006·Environmental Health Perspectives·Ioanna KatsiadakiAlexander Pickering Scott
Mar 10, 2005·Analytical and Bioanalytical Chemistry·Juan F AsturianoAngel Maquieira
Mar 5, 2008·Analytical and Bioanalytical Chemistry·Maoyong SongGuibin Jiang
Mar 10, 2001·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·J F TolarR A Angus
Oct 16, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Christy M ForanWilliam H Benson
Feb 26, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Eve MylchreestGerald T Ankley
Dec 9, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Susan C TiltonWilliam H Benson
Oct 21, 2014·International Journal of Molecular Sciences·Hee Jeong KongCheul Min An
Jan 1, 2005·Toxicology Mechanisms and Methods·James R WheelerDavid Morritt
Dec 31, 2002·Environmental Toxicology and Chemistry·Robert B Bringolf, Robert C Summerfelt
Jan 11, 2011·Aquatic Toxicology·Bethany R HannasGerald A Leblanc
Jan 18, 2011·Journal of Environmental Sciences (China)·Mário S DinizFernando Santana
Aug 5, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Jennifer A Shelby-WalkerMary T Mendonça
Oct 18, 2011·Environmental Toxicology and Chemistry·Richard D HandyNina Horne
May 15, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Hua TianWei Wang
Nov 1, 2008·Toxicology and Applied Pharmacology·Iurgi SalaberriaBjørn Munro Jenssen
Nov 22, 2007·Environment International·Valerio MatozzoChristian Blaise
Aug 11, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Om PrakashNeeta Sehgal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.