Universal initiator nucleotides for the enzymatic synthesis of 5'-amino- and 5'-thiol-modified RNA

Biochemical and Biophysical Research Communications
Jörg C Schlatterer, A Jäschke

Abstract

We report the chemical synthesis of 5'-amino- and 5'-thiol-hexaethylene glycol guanosine nucleotides and their enzymatic incorporation into RNA, followed by chemical modifications at their nucleophilic ends. By using two similar routes, the conjugates of guanosine-5'-monophosphate and hexaethylene glycol with attached reactive groups (SH or NH(2)) were synthesized using phosphoramidite chemistry, and characterized by MALDI TOF mass spectrometry. These initiator molecules were efficiently incorporated into RNA at the 5'-end by run-off transcription using T7 RNA polymerase. The potential of these RNA conjugates for a broad reaction range with electrophiles is shown here, thereby enabling their use for diverse biochemical applications.

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Citations

Jan 11, 2012·Biochemical and Biophysical Research Communications·Florentine Wahl, Andres Jäschke
Oct 12, 2013·Angewandte Chemie·Ugo PradèreJonathan Hall
Sep 1, 2012·Angewandte Chemie·Helen LewisIan C Eperon
Feb 23, 2008·Organic & Biomolecular Chemistry·Jörn WolfSabine Müller
Apr 13, 2019·Chemical Communications : Chem Comm·Hyun Shin ParkEric T Kool
Feb 7, 2020·Chemical Communications : Chem Comm·Nikolaos PapastavrouPiet Herdewijn

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