Universal label-free in-process quantification of influenza virus-like particles

Biotechnology Journal
Sofia B CarvalhoManuel J T Carrondo

Abstract

Virus-like particles (VLPs) are becoming established as vaccines, in particular for influenza pandemics, increasing the interest in the development of VLPs manufacturing bioprocess. However, for complex VLPs, the analytical tools used for quantification are not yet able to keep up with the bioprocess progress. Currently, quantification for Influenza relies on traditional methods: hemagglutination assay or Single Radial Immunodiffusion. These analytical technologies are time-consuming, cumbersome, and not supportive of efficient downstream process development and monitoring. Hereby we report a label-free tool that uses Biolayer interferometry (BLI) technology applied on an Octet platform to quantify Influenza VLPs at all stages of bioprocess. Human (α2,6-linked sialic acid) and avian (α2,3-linked sialic acid) biotinylated receptors associated with streptavidin biosensors were used, to quantify hemagglutinin content in several mono- and multivalent Influenza VLPs. The applied method was able to quantify hemagglutinin from crude samples up to final bioprocessing VLP product. BLI technology confirmed its value as a high throughput analytical tool with high sensitivity and improved detection limits compared to traditional methods. T...Continue Reading

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Citations

Jan 1, 2019·Biotechnology and Bioengineering·Hayley K Charlton HumeLinda H L Lua
Jul 17, 2018·Journal of Chemical Technology and Biotechnology·Sofia B CarvalhoManuel Jt Carrondo
May 21, 2019·Biotechnology Journal·Sofia B CarvalhoCristina Peixoto
Mar 16, 2021·ACS Omega·Tamsyn StanboroughDeborah L Crittenden
Mar 20, 2021·Molecular Therapy. Methods & Clinical Development·Andreas L GimpelRichard D Braatz
Apr 30, 2021·Biotechnology and Bioengineering·Sofia B CarvalhoRicardo J S Silva

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