Universal light-sheet generation with field synthesis

Nature Methods
Bo-Jui ChangReto Fiolka

Abstract

We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.

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Citations

Sep 11, 2020·Optics Express·Bo-Jui ChangReto Fiolka
Feb 6, 2020·Biomedical Optics Express·Elena RemachaFlorian O Fahrbach
Mar 20, 2019·Nature Methods·Manu Forero-Shelton
Dec 20, 2019·Biomedical Optics Express·Leon van der GraaffSjoerd Stallinga
Mar 3, 2020·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Suhui DengMingping Liu
Dec 3, 2020·Nature Communications·Mingxing ZhangAndreas Gahlmann
Nov 6, 2020·Biomedical Optics Express·Vincent MaioliWilly Supatto
May 23, 2021·Nature Protocols·Kurt R WeissJan Huisken
Jun 30, 2021·Nature Methods·Bo-Jui ChangReto Fiolka
Dec 1, 2019·Nanophotonics·Jialei TangKyu Young Han
Oct 13, 2021·Nature Biotechnology·Bin CaoAlexandros Pertsinidis
Oct 12, 2021·Frontiers in Neuroanatomy·Zhouzhou ZhangZengcai V Guo
Jan 16, 2022·Cell Biology International·Mengdi ZhangJing Liu

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Methods Mentioned

BETA
Confocal microscopy
fluorescence microscopy
biosensor
chipset
FCS

Software Mentioned

mSPIM
MATLAB
MATLAB Live
Fiji
ImageJ
LabView
LLSM

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