Unlike catalyzing error-free bypass of 8-oxodGuo, DNA polymerase λ is responsible for a significant part of Fapy·dG-induced G → T mutations in human cells

Biochemistry
Paritosh PandeAshis K Basu

Abstract

8-OxodGuo and Fapy·dG induced 10-22% mutations, predominantly G → T transversions, in human embryonic kidney 293T cells in four TG*N sequence contexts, where N = C, G, A, or T. siRNA knockdown of pol λ resulted in 34 and 55% increases in the level of mutations in the progeny from the 8-oxodGuo construct in the TG*T and TG*G sequences, respectively, suggesting that pol λ is involved in error-free bypass of 8-oxodGuo. For Fapy·dG, in contrast, the level of G → T mutations was reduced by 27 and 46% in the TG*T and TG*G sequences, respectively, suggesting that pol λ is responsible for a significant fraction of Fapy·dG-induced G → T mutations.

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Citations

Sep 2, 2016·Biochemistry·Safnas F AbdulSalamEdward J Merino
Nov 2, 2016·Chemical Research in Toxicology·Ashis K BasuArindam Bose
Apr 25, 2017·Environmental and Molecular Mutagenesis·Yan ShaR Stephen Lloyd
Aug 3, 2016·The EMBO Journal·Matthew J BurakMiguel Garcia-Diaz
Jan 1, 2015·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Hiroyuki KamiyaIchiro Matsuoka
Dec 17, 2016·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Tetsuya Suzuki, Hiroyuki Kamiya
Jun 27, 2018·Journal of the American Chemical Society·Junzhou WuShana J Sturla

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