Unlinking of cell division from deoxyribonucleic acid replication in a temperature-sensitive deoxyribonucleic acid synthesis mutant of Escherichia coli.

Journal of Bacteriology
M Inouye

Abstract

A new type of temperature-sensitive deoxyribonucleic acid (DNA) synthesis mutant, which can divide without a completion of DNA replication, was isolated from a thymidine-requiring Escherichia coli strain by means of photo-bromouracil selection after nitrosoguanidine mutagenesis. In this mutant, in spite of the fact that DNA synthesis stopped immediately after the temperature shift from 30 to 41 C, cells could continue to divide, though at a reduced rate. This cell division without DNA synthesis at 41 C is further supported by the following results. (i) Cell division took place at high temperature without addition of thymidine but not at all at 30 C. The parent strain of the mutant did not divide at 41 C without thymidine. (ii) Smaller cells isolated from the culture grown at 41 C did not contain DNA. This was shown by chemical analysis of the smaller cells and on electron micrographs. Ability of cells to divide was examined according to sizes of cells. By using the culture at 30 C, cells of various sizes were separated by means of sucrose-density gradient centrifugation. It was found that all cell fractions, including the smallest one, could divide at high temperature. These results suggest that in this mutant the completion of...Continue Reading

References

Feb 14, 1968·Journal of Molecular Biology·S Cooper, C E Helmstetter
Feb 1, 1968·Journal of Bacteriology·E Dubnau, W K Maas
May 1, 1968·Journal of Bacteriology·C E Helmstetter, O Pierucci
Jan 1, 1969·Journal of Bacteriology·J V BoyleW A Goss
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·Y HirotaF Jacob
Oct 14, 1968·Journal of Molecular Biology·H S Smith, L I Pizer
May 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·E M Witkin
May 4, 1965·Biochimica Et Biophysica Acta·L S PRESTIDGE, A B PARDEE
Feb 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·H I AdlerA A Hardigree

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Citations

Jan 1, 1974·Molecular & General Genetics : MGG·A Toussaint, M Faelen
Jan 1, 1972·Journal of Supramolecular Structure·B HoffmannU Schwarz
Feb 21, 1974·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R H Pritchard
Jan 1, 1970·Molecular & General Genetics : MGG·P L Carl
Jan 1, 1971·Molecular & General Genetics : MGG·J A Wechsler, J D Gross
Jun 30, 1971·Nature: New Biology·W D DonachieK J Begg
May 1, 1971·CRC Critical Reviews in Microbiology·M L Higgins, G D Shockman
Oct 24, 1977·Molecular & General Genetics : MGG·M G Sargent
Jan 1, 1972·Molecular & General Genetics : MGG·Z CieślaT Klopotowski
Dec 17, 1971·Nature·A Tomasz
Feb 15, 1977·Molecular & General Genetics : MGG·S L Travis, N H Mendelson
Jan 1, 1973·Progress in Biophysics and Molecular Biology·D W Smith
Mar 1, 1980·Journal of Bacteriology·M S Tang, C E Helmstetter
Sep 1, 1973·Journal of Bacteriology·D E Dix, C E Helmstetter
May 1, 1973·Journal of Bacteriology·J N ReeveR M Cole
May 1, 1973·Journal of Bacteriology·P C Wu, A B Pardee
Mar 1, 1978·Journal of Bacteriology·G Satta, A B Pardee
Jan 1, 1978·Journal of Bacteriology·J A Sturgeon, L O Ingram
Dec 1, 1974·Mutation Research·J Greenberg, J Donch
Jun 1, 1974·Bacteriological Reviews·M Slater, M Schaechter
Jun 1, 1970·Bacteriological Reviews·H J Rogers
Mar 26, 2003·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Teruyo ItoKeiichi Hiramatsu
Nov 1, 1972·Journal of Bacteriology·N H Mendelson, R M Cole
Jul 25, 1975·Journal of Molecular Biology·S Silberstein, M Inouye
Aug 14, 1972·Journal of Molecular Biology·D R ZusmanA B Pardee

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