Dec 10, 2016

Unlocking bat immunology: establishment of Pteropus alecto bone marrow-derived dendritic cells and macrophages

Scientific Reports
Peng ZhouLin-Fa Wang

Abstract

Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages. Cells with the phenotype, morphology and functional features of monocyte-derived DC, bona fide DC or macrophages were obtained in GM-CSF/IL4, FLT3L or CSF-1 cultures, respectively. The successful generation of the first bat bone marrow-derived immune cells paves the way to unlocking the immune mechanisms that confer host resilience to pathogens in bats.

  • References29
  • Citations1

References

Mentioned in this Paper

SARS2 gene
Severe Acute Respiratory Syndrome
Immune Response
T-Lymphocyte
Immune Effector Cell
Virus Diseases
Ebola virus
FLT3LG gene
Granulocyte Colony-Stimulating Factor
Ebolavirus

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