Unlocking biodiversity and conservation studies in high-diversity environments using environmental DNA (eDNA): A test with Guianese freshwater fishes

Molecular Ecology Resources
Kévin CillerosSébastien Brosse

Abstract

Determining the species compositions of local assemblages is a prerequisite to understanding how anthropogenic disturbances affect biodiversity. However, biodiversity measurements often remain incomplete due to the limited efficiency of sampling methods. This is particularly true in freshwater tropical environments that host rich fish assemblages, for which assessments are uncertain and often rely on destructive methods. Developing an efficient and nondestructive method to assess biodiversity in tropical freshwaters is highly important. In this study, we tested the efficiency of environmental DNA (eDNA) metabarcoding to assess the fish diversity of 39 Guianese sites. We compared the diversity and composition of assemblages obtained using traditional and metabarcoding methods. More than 7,000 individual fish belonging to 203 Guianese fish species were collected by traditional sampling methods, and ~17 million reads were produced by metabarcoding, among which ~8 million reads were assigned to 148 fish taxonomic units, including 132 fish species. The two methods detected a similar number of species at each site, but the species identities partially matched. The assemblage compositions from the different drainage basins were better...Continue Reading

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Citations

Mar 1, 2019·Scientific Reports·Isabel CanteraSébastien Brosse
Nov 2, 2019·Journal of Fish Biology·Tristan MilhauTony Dejean
Oct 22, 2019·Molecular Ecology Resources·Girish KumarMichelle R Gaither
Dec 12, 2019·Molecular Ecology Resources·Shawn R NarumBenjamin Sibbett
Sep 14, 2020·Molecular Ecology·Gentile Francesco FicetolaPierre Taberlet
Oct 9, 2019·Biodiversity Data Journal·Jérôme MurienneSébastien Brosse
Jun 11, 2020·Molecular Biology Reports·Priscila MezzomoRogério L Cansian
Aug 14, 2020·Molecular Ecology·Carla Martins LopesKelly Raquel Zamudio
Feb 1, 2019·Molecular Ecology Resources·Christopher L JerdeTerra L Dressler
Nov 27, 2019·Journal of Fish Biology·Christopher L Jerde
Oct 16, 2020·The Science of the Total Environment·Shuping WangJianlong Li
Oct 23, 2020·Scientific Reports·David T MilanDaniel C Carvalho
Apr 1, 2021·Molecular Ecology Resources·Opale CoutantSébastien Brosse
May 16, 2021·Scientific Reports·Rosetta C BlackmanFlorian Altermatt
Aug 10, 2021·Marine Pollution Bulletin·Vignesh Kumar BalasubramanianRamesh Ramachandran
Nov 2, 2021·Molecular Ecology Resources·Isabel CanteraSébastien Brosse

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