Unlocking the diversity of alkaloids in Catharanthus roseus: nuclear localization suggests metabolic channeling in secondary metabolism

Chemistry & Biology
Anna StavrinidesSarah E O'Connor

Abstract

The extraordinary chemical diversity of the plant-derived monoterpene indole alkaloids, which include vinblastine, quinine, and strychnine, originates from a single biosynthetic intermediate, strictosidine aglycone. Here we report for the first time the cloning of a biosynthetic gene and characterization of the corresponding enzyme that acts at this crucial branchpoint. This enzyme, an alcohol dehydrogenase homolog, converts strictosidine aglycone to the heteroyohimbine-type alkaloid tetrahydroalstonine. We also demonstrate how this enzyme, which uses a highly reactive substrate, may interact with the upstream enzyme of the pathway.

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Citations

Jan 17, 2016·Current Opinion in Chemical Biology·Hajo Kries, Sarah E O'Connor
Jan 10, 2016·Current Opinion in Biotechnology·Sabrina Schläger, Birgit Dräger
Jun 25, 2016·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Matthew B Kilgore, Toni M Kutchan
Mar 12, 2015·The Plant Journal : for Cell and Molecular Biology·Franziska KellnerSarah E O'Connor
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Jul 20, 2017·Chembiochem : a European Journal of Chemical Biology·Sebastian RothMichael Müller
Feb 10, 2018·Chembiochem : a European Journal of Chemical Biology·Anna K StavrinidesSarah E O'Connor
May 19, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Hafiza Rida RiazBilal Haider Abbasi
Nov 20, 2018·Journal of Experimental Botany·Anna B DommesAnnette Becker
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Feb 12, 2021·Scientific Reports·Li-Qun ChenSixue Chen
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Jul 23, 2021·Journal of Experimental Botany·Sayantan PandaAsaph Aharoni
Jul 13, 2019·Journal of the American Chemical Society·Jin GuoKatherine S Ryan

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Datasets Mentioned

BETA
KM524258

Methods Mentioned

BETA
RNA-seq
NMR
isothermal titration calorimetry
bimolecular fluorescence complementation
PCR
fluorescence imaging
pulldown

Software Mentioned

BLAST

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