PMID: 36147May 17, 1979

Unmasking of a potassium leak in resealed human red blood cell ghosts

Biochimica Et Biophysica Acta
P G Wood, U Rossleben

Abstract

A selective potassium leak is observed in resealed, human red blood cell ghosts when hemolysis is performed with distilled water at pH 6.5, 0 degrees C. The leak, which has a maximum near pH 6.7, is suppressed when either magnesium or a chelating agent is present in the hemolysing medium. The potassium leak has the additional property that it can be suppressed after resealing by washing the ghost membranes in a medium containing a low concentration of ATP or EDTA. The data suggest that through the dilution of endogenous chelating agents at hemolysis a potassium leak may be unmasked.

References

Dec 4, 1975·The Journal of Membrane Biology·P A KnaufH Passow
Nov 29, 1976·The Journal of Membrane Biology·P J Romero
May 1, 1952·The Journal of General Physiology·T TEORELL

Related Concepts

Cell Membrane Permeability
Metal Antagonists
Red Cell Ghost
Erythrocytes
Hemolysis
Hydrogen-Ion Concentration
Magnesium
Potassium

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