Unprecedented hour-long residence time of a cation in a left-handed G-quadruplex.

Chemical Science
Fernaldo Richtia WinnerdyAnh Tuân Phan

Abstract

Cations are critical for the folding and assembly of nucleic acids. In G-quadruplex structures, cations can bind between stacked G-tetrads and coordinate with negatively charged guanine carbonyl oxygens. They usually exchange between binding sites and with the bulk in solution with time constants ranging from sub-millisecond to seconds. Here we report the first observation of extremely long-lived K+ and NH4+ ions, with an exchange time constant on the order of an hour, when coordinated at the center of a left-handed G-quadruplex DNA. A single-base mutation, that switched one half of the structure from left- to right-handed conformation resulting in a right-left hybrid G-quadruplex, was shown to remove this long-lived behaviour of the central cation.

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Citations

Sep 22, 2021·Accounts of Chemical Research·Valérie Gabelica
Oct 1, 2021·Chemical Reviews·Eric LargyValérie Gabelica

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