Unraveling the binding interaction and kinetics of a prospective anti-HIV drug with a model transport protein: results and challenges

Physical Chemistry Chemical Physics : PCCP
Bijan Kumar PaulNikhil Guchhait

Abstract

The present contribution reports a detailed characterization of the binding interaction of a potential anticancer, anti-HIV drug 1-phenylisatin (1-PI) with a model transport protein Bovine Serum Albumin (BSA) using fluorescence spectroscopic techniques. The thermodynamic parameters e.g., ΔH, ΔS and ΔG for the binding phenomenon have been evaluated on the basis of the van't Hoff equation to reveal that the binding process is principally driven by ionic interactions mediated by charge transfer interaction. This line of argument has been substantiated by frontier molecular orbital analysis of 1-PI. However, the drug-induced quenching of the intrinsic tryptophanyl fluorescence of the protein is found not to abide by a linear Stern-Volmer regression (displaying an upward curvature) when an extensive time-resolved fluorescence spectroscopic characterization of the quenching process has been undertaken to unveil the actuating quenching mechanism. Based on the constancy of the fluorescence lifetime of the protein as a function of drug concentration the observed quenching is inferred to proceed through a static mechanism between the quenching partners. Constant wavelength synchronous fluorescence, excitation-emission matrix fluorescence...Continue Reading

References

Apr 1, 1979·Journal of Pharmaceutical Sciences·M KupinićD Maysinger
Jul 16, 1992·Nature·X M He, D C Carter
Mar 24, 1989·Biochimica Et Biophysica Acta·A PalumboG Prota
Jan 1, 1985·Annual Review of Biochemistry·J M Beechem, L Brand
Jan 1, 1994·Advances in Protein Chemistry·D C Carter, J X Ho
May 28, 1993·Science·R A Weiss
May 6, 1997·Biochemistry·S BanerjeeB Bhattacharyya
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·D ZhongA H Zewail
Jul 18, 2001·Journal of the American Chemical Society·R J ChenH Dai
Aug 2, 2001·Nature Biotechnology·J Hodgson
Jul 2, 2003·Current Opinion in Structural Biology·Stephen J CampbellDavid R Westhead
Dec 7, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Robert J MotzerBrian I Rini
Feb 8, 2006·FEBS Letters·Csaba Hetényi, David van der Spoel
Apr 28, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hans PrenenMaria Debiec-Rychter
Dec 30, 2006·Journal of Enzyme Inhibition and Medicinal Chemistry·Suresh RathinasamyUpal Kanti Mazumder
Apr 5, 2007·Biomacromolecules·Tuoqi WuZejian Cai
Aug 29, 2007·Analytica Chimica Acta·Victor C DiculescuAna Maria Oliveira-Brett
Oct 25, 2008·Annual Review of Medicine·Daniel C DouekRichard A Koup
Jan 29, 2009·Biomacromolecules·Yan-Jun HuXiao-He Xiao
Feb 10, 2009·The Journal of Physical Chemistry. B·Bhaswati BhattacharyaAnunay Samanta
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
May 16, 2009·Anti-cancer Agents in Medicinal Chemistry·K L VineD Skropeta
Nov 11, 2009·Biomacromolecules·Yan-Jun HuXiao-He Xiao
Feb 2, 2010·Journal of Molecular Biology·Ernesto A RomanF Luis González Flecha
Sep 4, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Lennart TreuelReinhard Zellner
Jan 12, 2011·Journal of Fluorescence·María Del Carmen PintoPedro Macías
Mar 5, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bijan Kumar Paul, Nikhil Guchhait
Sep 17, 2011·The Journal of Physical Chemistry. B·Yang ShuJianhua Wang
Oct 18, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Kotni SanthoshAnunay Samanta
Jan 24, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bijan Kumar Paul, Nikhil Guchhait
Feb 23, 2012·Physical Chemistry Chemical Physics : PCCP·Uttam AnandSaptarshi Mukherjee

❮ Previous
Next ❯

Citations

Mar 29, 2014·Physical Chemistry Chemical Physics : PCCP·Aniruddha GangulyNikhil Guchhait
Apr 6, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chunyan LiShuanli Guo
Nov 18, 2015·Journal of Liposome Research·Peter HinowIan G Tucker
Jan 12, 2016·Physical Chemistry Chemical Physics : PCCP·Jing WangRutao Liu
Apr 7, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Pablo F GarridoÁngel Piñeiro
Jul 15, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Khan Behlol Ayaz AhmedAnbazhagan Veerappan
Sep 22, 2015·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Yunfeng ZhaiKaren Swider-Lyons
May 28, 2013·Biochimica Et Biophysica Acta·Uttam Anand, Saptarshi Mukherjee
Jul 7, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Narayani GhoshSaptarshi Mukherjee
Jul 29, 2015·The Journal of Physical Chemistry. B·Robert SeidelBernd Winter
Aug 31, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ran MiAi-Min Bai
Jan 24, 2015·Journal of Colloid and Interface Science·Xiaoqing XuChenxin Cai
Aug 12, 2015·The Journal of Physical Chemistry Letters·Zlatko BrkljačaAna-Sunčana Smith
Mar 19, 2014·The Journal of Physical Chemistry. B·Manjumol MathewUsha K Aravind
May 9, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Bijan K PaulSaptarshi Mukherjee
Nov 14, 2016·International Journal of Biological Macromolecules·Fatemeh S Mohseni-ShahriHooriyeh Nassirli
Mar 25, 2017·ACS Applied Materials & Interfaces·Ali OthmanSilvana Andreescu
Apr 15, 2018·Journal of Molecular Recognition : JMR·Lining ZhaoRutao Liu
Aug 21, 2018·Journal of Molecular Recognition : JMR·Xun ZhangRutao Liu
Oct 29, 2018·Journal of Biochemical and Molecular Toxicology·Jing WangWansong Zong
Jun 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Samuel K KwofieMichael D Wilson
Feb 7, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Lining ZhaoRutao Liu
Sep 26, 2013·Physical Chemistry Chemical Physics : PCCP·Vincent GoovaertsTatjana N Parac-Vogt
Jan 28, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Amit AkhuliMoloy Sarkar
May 4, 2017·Food Research International·Cen ZhangDeming Gong
May 25, 2021·Diamond and Related Materials·Kamal A Soliman, S Abdel Aal
Jan 11, 2020·The Journal of Physical Chemistry. B·Mullah Muhaiminul IslamMoloy Sarkar
Jun 15, 2018·Journal of Agricultural and Food Chemistry·Huafang DingGuowen Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.