Unraveling the folding mechanism of the smallest knotted protein, MJ0366

The Journal of Physical Chemistry. B
Iren WangShang-Te Danny Hsu

Abstract

Understanding the mechanism by which polypeptide chains thread themselves into topologically knotted structures has emerged to be a challenging subject not least because of the additional complexity associated with the spontaneous and efficient knotting and folding events. While recent theoretical calculations have made significant progress in establishing the atomistic folding pathways for a number of knotted proteins, experimental data on the folding stabilities and kinetic pathways of knotted proteins has been sparse. Using MJ0366 from Methanocaldococcus jannaschii, the smallest knotted protein known to date, as a model system, we set out to systematically investigate its folding equilibrium, kinetics, and internal dynamics under native and chemically denatured states. NMR hydrogen-deuterium exchange analysis indicates that the knotted region is the most stable structural element within the novel fold. Additionally, (15)N spin relaxation analysis reveals the presence of residual structures in urea-denatured MJ0366. Despite the apparent two-state equilibrium unfolding behavior during chemical denaturation, the kinetic unfolding pathway of MJ0366 involves the dissociation of the homodimeric native state into a native-like mono...Continue Reading

References

Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Jan 1, 1996·Folding & Design·R L Baldwin
Mar 2, 2002·Science·Robert L Baldwin
Mar 2, 2002·Science·Judith Klein-SeetharamanHarald Schwalbe
Jul 22, 2004·Journal of the American Chemical Society·Alexander P GolovanovLu-Yun Lian
Jul 31, 2004·Methods : a Companion to Methods in Enzymology·Mallela M G KrishnaS Walter Englander
Jan 12, 2005·Biochemistry·Jia-Cherng HorngDaniel P Raleigh
Feb 17, 2005·Journal of Molecular Biology·Anna L Mallam, Sophie E Jackson
May 13, 2006·The FEBS Journal·Tapan K Chaudhuri, Subhankar Paul
May 30, 2006·Protein Science : a Publication of the Protein Society·Julia WirmerHarald Schwalbe
Jun 22, 2006·Journal of Molecular Biology·Anna L Mallam, Sophie E Jackson
Sep 19, 2006·PLoS Computational Biology·Peter VirnauMehran Kardar
Dec 16, 2006·Journal of Molecular Biology·Anna L Mallam, Sophie E Jackson
Mar 21, 2007·Journal of Molecular Biology·Stefan WallinEugene I Shakhnovich
May 22, 2007·Journal of Molecular Biology·Jie-rong HuangSophie E Jackson
May 29, 2007·Nucleic Acids Research·Yan-Long LaiJenn-Kang Hwang
Dec 17, 2008·The FEBS Journal·Anna L Mallam
Feb 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Joanna I SułkowskaJosé Onuchic
May 30, 2009·The FEBS Journal·Fredrik I AnderssonSophie E Jackson
Jul 29, 2009·Biomolecular NMR Assignments·Shang-Te Danny HsuChristopher M Dobson
Nov 11, 2009·The Journal of Biological Chemistry·Shang-Te Danny HsuSophie E Jackson
Apr 7, 2010·Physical Review Letters·Joanna I SułkowskaJosé N Onuchic
Apr 7, 2010·PLoS Computational Biology·Daniel BölingerPeter Virnau
Apr 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anna L MallamSophie E Jackson
Aug 6, 2010·PLoS Computational Biology·Raffaello PotestioHenri Orland
Aug 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey K NoelJosé N Onuchic
Jan 22, 2011·Journal of Molecular Biology·Fredrik I AnderssonSophie E Jackson
Mar 17, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Peter VirnauSophie Jackson
Dec 20, 2011·Nature Chemical Biology·Anna L Mallam, Sophie E Jackson
Aug 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Joanna I SułkowskaJose N Onuchic
Apr 5, 2013·PLoS Computational Biology·Silvio a BeccaraPietro Faccioli
Jul 4, 2013·Biomolecular NMR Assignments·Shu-Ju Micky HsiehShang-Te Danny Hsu
Jun 21, 2014·Trends in Cell Biology·Mark S HippF Ulrich Hartl

❮ Previous
Next ❯

Citations

Jun 25, 2016·Proceedings of the National Academy of Sciences of the United States of America·Fabian ZieglerMatthias Rief
Aug 17, 2016·Scientific Reports·Iren WangShang-Te Danny Hsu
Oct 30, 2016·Current Opinion in Structural Biology·Sophie E JacksonCristian Micheletti
Dec 17, 2016·Scientific Reports·Shang-Te Danny Hsu
Feb 9, 2017·Protein Science : a Publication of the Protein Society·María Paz RamírezChristian A M Wilson
Mar 12, 2017·Biomolecular NMR Assignments·David J Burban, Patricia A Jennings
Aug 17, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chih-Ta Henry ChienShang-Te Danny Hsu
Oct 11, 2016·Physical Chemistry Chemical Physics : PCCP·Miguel A SolerPatrícia F N Faísca
Aug 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Álvaro San MartínMauricio Baez
Jul 4, 2019·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Claudio Perego, Raffaello Potestio
Feb 23, 2019·Scientific Reports·Elin M SivertssonLaura S Itzhaki
Aug 21, 2015·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Liang-Wei WangShang-Te Danny Hsu
Aug 21, 2015·Journal of Physics. Condensed Matter : an Institute of Physics Journal·P Dabrowski-TumanskiJ I Sulkowska
Sep 16, 2017·Polymers·Pawel Dabrowski-Tumanski, Joanna I Sulkowska
Feb 18, 2020·Current Opinion in Structural Biology·Joanna Ida Sulkowska
Apr 5, 2021·Biophysical Journal·Cristina PaissoniShang-Te Danny Hsu
Jun 11, 2021·Frontiers in Chemistry·Shang-Te Danny HsuHideo Iwaï
Apr 8, 2015·The Journal of Physical Chemistry. B·Po-Min ShihShang-Te Danny Hsu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.