Unraveling the function of Arabidopsis thaliana OS9 in the endoplasmic reticulum-associated degradation of glycoproteins.

Plant Molecular Biology
Silvia HüttnerRichard Strasser

Abstract

In the endoplasmic reticulum, immature polypeptides coincide with terminally misfolded proteins. Consequently, cells need a well-balanced quality control system, which decides about the fate of individual proteins and maintains protein homeostasis. Misfolded and unassembled proteins are sent for destruction via the endoplasmic reticulum-associated degradation (ERAD) machinery to prevent the accumulation of potentially toxic protein aggregates. Here, we report the identification of Arabidopsis thaliana OS9 as a component of the plant ERAD pathway. OS9 is an ER-resident glycoprotein containing a mannose-6-phosphate receptor homology domain, which is also found in yeast and mammalian lectins involved in ERAD. OS9 fused to the C-terminal domain of YOS9 can complement the ERAD defect of the corresponding yeast Δyos9 mutant. An A. thaliana OS9 loss-of-function line suppresses the severe growth phenotype of the bri1-5 and bri1-9 mutant plants, which harbour mutated forms of the brassinosteroid receptor BRI1. Co-immunoprecipitation studies demonstrated that OS9 associates with Arabidopsis SEL1L/HRD3, which is part of the plant ERAD complex and with the ERAD substrates BRI1-5 and BRI1-9, but only the binding to BRI1-5 occurs in a glycan...Continue Reading

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Citations

Jan 22, 2013·Annual Review of Plant Biology·Stephen H Howell
Sep 16, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yidan LiuJianming Li
Feb 26, 2016·Glycobiology·Richard Strasser
Mar 30, 2016·Plant Reproduction·Sotirios FragkostefanakisEnrico Schleiff
Sep 24, 2015·Plant Science : an International Journal of Experimental Plant Biology·Nausicaä Lannoo, Els J M Van Damme
Sep 19, 2014·The Plant Journal : for Cell and Molecular Biology·Jennifer SchobererRichard Strasser
May 10, 2015·Applied Microbiology and Biotechnology·Dezhong WangXiaokun Li
May 13, 2014·Frontiers in Plant Science·Yidan Liu, Jianming Li
Dec 13, 2016·The Plant Journal : for Cell and Molecular Biology·Evan AngelosFederica Brandizzi
May 10, 2017·The Plant Journal : for Cell and Molecular Biology·Hui XueGeorg J Seifert
Feb 6, 2018·The Plant Journal : for Cell and Molecular Biology·Yun-Ji ShinRichard Strasser
Apr 18, 2013·International Journal of Molecular Sciences·Yan DengStephen H Howell
Mar 24, 2018·Annual Review of Plant Biology·Richard Strasser
Feb 4, 2019·The Plant Journal : for Cell and Molecular Biology·Rongxue ZhangTao Wang
Sep 21, 2017·Frontiers in Plant Science·Md Kamrul HasanZhen-Yu Qi
Jan 20, 2019·International Journal of Molecular Sciences·Agata K ZupanskaAnna-Lisa Paul
Dec 16, 2019·The New Phytologist·Qian ChenQi Xie
Mar 19, 2016·The New Phytologist·Jian-Xiang Liu, Stephen H Howell
Aug 7, 2018·Plant Science : an International Journal of Experimental Plant Biology·Yukihiro NagashimaHisashi Koiwa
Mar 9, 2021·Frontiers in Plant Science·Richard StrasserJosé M Estevez

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Methods Mentioned

BETA
glycosylation
genotyping
PCR
transgenic
salt treatments
protein folding

Software Mentioned

ClustalW
Quantity One

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