Unraveling the global microRNAome responses to ionizing radiation in human embryonic stem cells.

PloS One
Mykyta V SokolovRonald D Neumann

Abstract

MicroRNAs (miRNA) comprise a group of short ribonucleic acid molecules implicated in regulation of key biological processes and functions at the post-transcriptional level. Ionizing radiation (IR) causes DNA damage and generally triggers cellular stress response. However, the role of miRNAs in IR-induced response in human embryonic stem cells (hESC) has not been defined yet. Here, by using system biology approaches, we show for the first time, that miRNAome undergoes global alterations in hESC (H1 and H9 lines) after IR. Interrogation of expression levels of 1,090 miRNA species in irradiated hESC showed statistically significant changes in 54 genes following 1 Gy of X-ray exposures; global miRNAome alterations were found to be highly temporally and cell line--dependent in hESC. Time-course studies showed that the 16 hr miRNAome radiation response of hESC is much more robust compared to 2 hr-response signature (only eight genes), and may be involved in regulating the cell cycle. Quantitative real-time PCR performed on some miRNA species confirms the robustness of our miRNA microarray platform. Positive regulation of differentiation-, cell cycle-, ion transport- and endomembrane system-related processes were predicted to be negat...Continue Reading

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Citations

Jan 9, 2014·International Journal of Molecular Sciences·Mykyta Sokolov, Ronald Neumann
Nov 12, 2013·Antioxidants & Redox Signaling·Jennifer R Czochor, Peter M Glazer
Feb 26, 2014·Mutation Research. Reviews in Mutation Research·Katrin MandaGuido Hildebrandt
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Jan 23, 2015·Bioinformatics·Thomas BleazardSam Griffiths-Jones
Dec 17, 2016·International Journal of Molecular Sciences·Eva Yi KongKwan Ngok Yu
Aug 1, 2013·International Journal of Molecular Sciences·Mykyta Sokolov, Ronald Neumann
Mar 21, 2018·International Journal of Radiation Biology·Kim Ngan Tran, Jong-Il Choi
Nov 27, 2014·World Journal of Stem Cells·Mykyta V Sokolov, Ronald D Neumann
Dec 12, 2012·International Journal of Molecular Sciences·Kai-Fu Tang, Hong Ren
May 26, 2018·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Molykutty J AryankalayilC Norman Coleman

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Methods Mentioned

BETA
X-ray
transfection

Software Mentioned

ArrayTools
BRB
DAVID
MiRanda
Database for Annotation , Visualization and Integrated Discove...

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