Unraveling the Motions behind Enterovirus 71 Uncoating

Biophysical Journal
Caroline RossC Atilgan

Abstract

Enterovirus 71 can be a severe pathogen in small children and immunocompromised adults. Virus uncoating is a critical step in the infection of the host cell; however, the mechanisms that control this process remain poorly understood. We applied normal mode analysis and perturbation response scanning to several complexes of the virus capsid and present a coarse-graining approach to analyze the full capsid. We show that our method offers an alternative to expressing the system as a set of rigid blocks and accounts for the interconnection between nodes within each subunit and protein interfaces across the capsid. In our coarse-grained approach, the modes associated with capsid expansion are captured in the first three nondegenerate modes and correspond to the changes observed in structural studies of the virus. We show that the resolution of the analysis may be modified without losing information on the global motions leading to uncoating. Perturbation response scanning revealed that a protomer cannot serve as a functional unit to explain deformations of the capsid. Instead, we define a pentamer as the minimum functional unit to investigate changes within the capsid. From the modal analysis and perturbation response scanning, we l...Continue Reading

Citations

Aug 2, 2019·Future Microbiology·Xingjian WenAnchun Cheng
Feb 6, 2020·International Journal of Molecular Sciences·Olivier Sheik AmamuddyOzlem Tastan Bishop
Jun 1, 2018·Bioinformatics·Caroline RossÖzlem Tastan Bishop
Nov 17, 2020·Frontiers in Molecular Biosciences·Haleh AbdizadehCanan Atilgan
May 13, 2021·The Journal of Physical Chemistry. B·Meryem ErenOzlem Keskin
Aug 28, 2021·Journal of Chemical Information and Modeling·Afrah KhairallahÖzlem Tastan Bishop

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