Unraveling the rationale behind organic solvent stability of lipases

Applied Biochemistry and Biotechnology
Debamitra ChakravortySanjukta Patra

Abstract

Organic solvent-stable lipases have pronounced impact on industrial economy as they are involved in synthesis by esterification, interesterification, and transesterification. However, very few of such natural lipases have been isolated till date. A study of the recent past provided few pillars to rely on for this work. The three-dimensional structure, inclusive of the surface and active site, of 29 organic solvent-stable lipases was analyzed by subfamily classification and protein solvent molecular docking based on fast Fourier transform correlation approach. The observations revealed that organic solvent stability of lipases is their intrinsic property and unique with respect to each lipase. In this paper, factors like surface distribution of charged, hydrophobic, and neutral residues, interaction of solvents with catalytically immutable residues, and residues interacting with essential water molecules required for lipase activity, synergistically and by mutualism contribute to render a stable lipase organic solvent. The propensity of surface charge in relation to stability in organic solvents by establishing repulsive forces to exclude solvent molecules from interacting with the surface and prohibiting the same from gaining e...Continue Reading

References

Jan 1, 1974·Advances in Protein Chemistry·I D Kuntz, W Kauzmann
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·S N Timasheff
Sep 23, 1998·Trends in Biotechnology·K E Jaeger, M T Reetz
Sep 28, 1998·Biochimica Et Biophysica Acta·K GekkoT Takagi
Feb 13, 2001·Enzyme and Microbial Technology·F CardenasE Alvarez
Dec 26, 2001·Acta Crystallographica. Section D, Biological Crystallography·Supachok SinchaikulMalcolm D Walkinshaw
May 4, 2004·BMC Bioinformatics·Shandar AhmadAkinori Sarai
Jul 1, 2004·International Journal of Biological Macromolecules·M Michael GromihaShandar Ahmad
May 24, 2005·Analytical Biochemistry·Raja Noor Zaliha R A RahmanAbu Bakar Salleh
Jun 9, 2005·Protein Expression and Purification·Hebin Li, Xiaobo Zhang
Oct 20, 2005·Journal of Bioscience and Bioengineering·H Ogino, H Ishikawa
Jun 14, 2006·Protein Expression and Purification·Moohamad Ropaning SulongMahiran Basri
Jan 4, 2007·BMC Bioinformatics·Gilles DidierIvan Laprevotte
Mar 21, 2007·Sheng wu gong cheng xue bao = Chinese journal of biotechnology·Zheng-Yu ShuYun-Jun Yan
Aug 9, 2007·BMC Bioinformatics·Abdellali KelilAlain Fleury
Oct 2, 2007·Biophysical Chemistry·Tsutomu ArakawaSerge N Timasheff
Nov 1, 1991·Biotechnology and Bioengineering·B Schulze, A M Klibanov
Nov 1, 1995·Talanta·M E Díaz-García, M J Valencia-González
Mar 10, 2009·Journal of Molecular Microbiology and Biotechnology·Bhawna Madan, Prashant Mishra
May 26, 2009·Nucleic Acids Research·Antonin PavelkaJiri Damborsky

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Citations

Oct 23, 2013·Molecular Biotechnology·Maryam Monsef ShokriKhosro Khajeh
Oct 7, 2015·Colloids and Surfaces. B, Biointerfaces·Keeran WardDavid C Stuckey
Jun 1, 2015·Applied Microbiology and Biotechnology·Adi DrorAyelet Fishman
Apr 30, 2014·The Protein Journal·Nor Hafizah Ahmad KamarudinAbu Bakar Salleh
Nov 29, 2013·Biotechnology and Bioengineering·Hyun Tae HwangArvind Varma
Mar 4, 2015·Bioresource Technology·Susan Hartwig DuarteFrancisco Valero
Oct 23, 2016·Biotechnology Progress·Solange S MagalhãesFilipe E Antunes
Feb 21, 2018·Biotechnology Journal·Marina LottiPau Ferrer
Jan 15, 2018·Extremophiles : Life Under Extreme Conditions·Yue ZhangYongming Bao
Feb 14, 2013·Chemical Society Reviews·Patrick Adlercreutz
Dec 12, 2018·Biotechnology Letters·Priyanka PriyankaBarry J Ryan

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