Unraveling the specific regulation of the central pathway for anaerobic degradation of 3-methylbenzoate.

The Journal of Biological Chemistry
Javier F JuárezEduardo Díaz

Abstract

The mbd cluster encodes the anaerobic degradation of 3-methylbenzoate in the β-proteobacterium Azoarcus sp. CIB. The specific transcriptional regulation circuit that controls the expression of the mbd genes was investigated. The PO, PB 1, and P3 R promoters responsible for the expression of the mbd genes, their cognate MbdR transcriptional repressor, as well as the MbdR operator regions (ATACN10GTAT) have been characterized. The three-dimensional structure of MbdR has been solved revealing a conformation similar to that of other TetR family transcriptional regulators. The first intermediate of the catabolic pathway, i.e. 3-methylbenzoyl-CoA, was shown to act as the inducer molecule. An additional MbdR-dependent promoter, PA, which contributes to the expression of the CoA ligase that activates 3-methylbenzoate to 3-methylbenzoyl-CoA, was shown to be necessary for an efficient induction of the mbd genes. Our results suggest that the mbd cluster recruited a regulatory system based on the MbdR regulator and its target promoters to evolve a distinct central catabolic pathway that is only expressed for the anaerobic degradation of aromatic compounds that generate 3-methylbenzoyl-CoA as the central metabolite. All these results highli...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Feb 1, 1997·Applied and Environmental Microbiology·P W Coschigano, L Y Young
Sep 25, 1998·The Journal of Biological Chemistry·A FerrándezE Díaz
Dec 12, 2001·Science·M A SchumacherR G Brennan
Jan 17, 2002·Journal of Biotechnology·Paul Gerhard RiegerHans Joachim Knackmuss
Feb 28, 2004·FEMS Microbiology Letters·Peter W Coschigano, Bradley J Bishop
Mar 26, 2004·Nature Reviews. Microbiology·Derek R Lovley
May 6, 2004·Archives of Microbiology·Barbara MoraschRainer U Meckenstock
Aug 20, 2004·Journal of Bacteriology·María J López BarragánEduardo Díaz
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Juan L RamosRaquel Tobes
Oct 24, 2007·Journal of Molecular Biology·Ming LiEdward W Yu
Apr 2, 2008·Annals of the New York Academy of Sciences·Georg Fuchs
Oct 11, 2008·Protein Expression and Purification·Huanting Liu, James H Naismith
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Manuel CarmonaEduardo Díaz
Apr 27, 2010·Journal of Structural and Functional Genomics·Muse OkeJames H Naismith
Sep 10, 2010·The Journal of Biological Chemistry·Gonzalo Durante-RodríguezManuel Carmona
Sep 24, 2010·Environmental Microbiology·Ramiro Vilchez-VargasDietmar H Pieper
Mar 15, 2011·Current Opinion in Biotechnology·Rainer U Meckenstock, Housna Mouttaki
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Jun 4, 2011·Bioengineered Bugs·Víctor de Lorenzo

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Methods Mentioned

BETA
PCR
X-ray
electrophoresis
Footprinting
in vitro transcription

Software Mentioned

CCP4
SEDNTERP
MATLAB
ClustalW
Quantity One
SEDFIT

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