Unraveling ultrafast photoinduced proton transfer dynamics in a fluorescent protein biosensor for Ca(2+) imaging

Chemistry : a European Journal
Longteng TangChong Fang

Abstract

Imaging Ca(2+) dynamics in living systems holds great potential to advance neuroscience and cellular biology. G-GECO1.1 is an intensiometric fluorescent protein Ca(2+) biosensor with a Thr-Tyr-Gly chromophore. The protonated chromophore emits green upon photoexcitation via excited-state proton transfer (ESPT). Upon Ca(2+) binding, a significant population of the chromophores becomes deprotonated. It remains elusive how the chromophore structurally evolves prior to and during ESPT, and how it is affected by Ca(2+) . We use femtosecond stimulated Raman spectroscopy to dissect ESPT in both the Ca(2+) -free and bound states. The protein chromophores exhibit a sub-200 fs vibrational frequency shift due to coherent small-scale proton motions. After wavepackets move out of the Franck-Condon region, ESPT gets faster in the Ca(2+) -bound protein, indicative of the formation of a more hydrophilic environment. These results reveal the governing structure-function relationship of Ca(2+) -sensing protein biosensors.

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Citations

Mar 17, 2016·The Journal of Physical Chemistry Letters·Longteng TangChong Fang
Mar 23, 2017·The Journal of Physical Chemistry. B·Longteng TangChong Fang
Feb 24, 2017·Physical Chemistry Chemical Physics : PCCP·Sean R TachibanaChong Fang
Oct 11, 2016·Physical Chemistry Chemical Physics : PCCP·Breland G OscarChong Fang
Sep 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Longteng TangChong Fang
Feb 23, 2020·Annual Review of Physical Chemistry·Chong Fang, Longteng Tang
May 1, 2018·Physical Chemistry Chemical Physics : PCCP·Longteng TangChong Fang
Apr 30, 2019·Physical Chemistry Chemical Physics : PCCP·Miles A TaylorChong Fang
Feb 27, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Daniel R Dietze, Richard A Mathies
Nov 30, 2019·The Journal of Chemical Physics·Chong FangCheng Chen
Aug 1, 2020·Frontiers in Molecular Biosciences·Breland G OscarChong Fang
Aug 17, 2018·The Journal of Physical Chemistry Letters·Longteng TangChong Fang
May 17, 2019·The Journal of Physical Chemistry. B·Longteng Tang, Chong Fang
Sep 27, 2018·The Journal of Physical Chemistry. a·Timothy J QuincyChristopher G Elles
Nov 20, 2018·The Journal of Physical Chemistry. B·Sean R TachibanaChong Fang

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