Unravelling the antigenic landscape of the HIV-1 subtype A envelope of an individual with broad cross-neutralizing antibodies using phage display peptide libraries

Journal of Virological Methods
Tessa DieltjensWouter Janssens

Abstract

Broad cross-neutralizing antibodies from persons infected with HIV-1 target a variety of epitopes. Identification of these HIV-1 epitopes may result in an optimal panel of antigenic peptides to be included in a prophylactic vaccine. Phage display peptide libraries were used to unravel the antigenic landscape of an individual (ITM1) infected with HIV-1 subtype A with broad cross-neutralizing antibodies. A stringent selection strategy resulted in the identification of 60 unique HIV-1 peptide phage, which were subjected to sequence analysis and mapped onto the ITM1 envelope sequences. Four groups of peptide phages were found: the first group (n=11) were similar with the tip of the V3 loop (KxxHxGPxxxF); the second group (n=11) represented the gp41 principal immunodominant domain (CxGxLxCTxNxP); the third group (n=16) could be localized in the V2 loop (KxxxHxxxY); and the fourth group (n=22) mimicked a conformational epitope (Hxx(S)/(T)NxK). All but the V2-binding antibodies were conserved over the 11 years of follow-up. A neutralization inhibition assay revealed the contribution of the V3 antibodies to the neutralizing capacity of the ITM1 plasma. Overall, the ITM1 immunogenic landscape was mapped and a part of the origin of this ...Continue Reading

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Citations

Feb 24, 2011·Journal of the American Chemical Society·Joan G SchellingerJacquelyn Gervay-Hague
Feb 8, 2013·Journal of Virology·Barbara C BachlerRuth M Ruprecht
Nov 13, 2012·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Xiaoli ZhangHong Shang
May 19, 2012·International Journal of Molecular Sciences·Sylvie DelhalleAndy Chevigné
Feb 23, 2019·Viruses·Michael L MalloryRalph S Baric
Nov 1, 2016·Biomedit︠s︡inskai︠a︡ khimii︠a︡·G A Kuzmicheva, V A Belyavskaya
May 16, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hannes UchtenhagenAnna-Lena Spetz

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