Abstract
Gene mutations conferring herbicide resistance are hypothesized to have negative pleiotropic effects on plant growth and fitness, which may in turn determine the evolutionary dynamics of herbicide resistance alleles. We used the widespread, annual, diploid grass weed Alopecurus aequalis as a model species to investigate the effect of two resistance mutations-the rare Pro-197-Tyr mutation and the most common mutation, Trp-574-Leu-on acetolactate synthase (ALS) functionality and plant growth. We characterized the enzyme kinetics of ALS from two purified A. aequalis populations, each homozygous for the resistance mutation 197-Tyr or 574-Leu, and assessed the pleiotropic effects of these mutations on plant growth. Both mutations reduced sensitivity of ALS to ALS-inhibiting herbicides without significant changes in extractable ALS activity. The 197-Tyr mutation slightly decreased the substrate affinity (corresponding to an increased Km for pyruvate) and maximum reaction velocity (Vmax) of ALS, whereas the 574-Leu mutation significantly increased these kinetics. Significant decrease or increase in plant growth associated, respectively, with the 197-Tyr and 574-Leu resistance mutations was highly correlated with their impact on ALS ki...Continue Reading
References
Aug 6, 2000·The Biochemical Journal·Y T Lee, R G Duggleby
Jul 20, 2002·Annals of Botany·William A Hoffmann, Hendrik Poorter
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jennifer A McCourtRonald G Duggleby
Jan 18, 2006·The New Phytologist·François J TardifMihai Costea
Aug 28, 2007·Plant Physiology·Qin YuStephen B Powles
Sep 15, 2007·The Journal of Biological Chemistry·Martha L Healy-FriedErnst Schönbrunn
Feb 1, 2008·Plant Physiology and Biochemistry : PPB·Ronald G DugglebyLuke W Guddat
Oct 15, 2009·The New Phytologist·Martin M Vila-AiubStephen B Powles
Mar 3, 2010·Annual Review of Plant Biology·Stephen B Powles, Qin Yu
Jul 16, 2010·Journal of Experimental Botany·Qin YuStephen B Powles
May 5, 2011·Heredity·M M Vila-AiubF Roux
Oct 26, 2012·Pest Management Science·Mei LiStephen B Powles
Jul 9, 2013·Trends in Genetics : TIG·Christophe DélyeValérie Le Corre
Jul 31, 2013·Pest Management Science·Kateřina HamouzováPavel Hamouz
Dec 18, 2013·Pest Management Science·Qin Yu, Stephen B Powles
May 29, 2015·Journal of Experimental Botany·Martin M Vila-AiubStephen B Powles
May 1, 2017·Pesticide Biochemistry and Physiology·Jordi Rey-CaballeroJoel Torra
Aug 30, 2017·Frontiers in Plant Science·Ning ZhaoJinxin Wang
Jan 10, 2018·Pest Management Science·Roman B VercellinoAlejandro Presotto
Feb 10, 2018·Pest Management Science·Qian YangMingqi Zheng
May 29, 2018·Pest Management Science·Ning ZhaoJinxin Wang
Jun 13, 2018·Pesticide Biochemistry and Physiology·Wenlei GuoJinxin Wang
Mar 9, 2019·Ecology and Evolution·Long DuJinxin Wang
May 8, 2019·PloS One·Yan LiPing Gong
Oct 2, 2019·Plants·Brent P Murphy, Patrick J Tranel
Nov 2, 2019·American Journal of Health Promotion : AJHP·Laurie Heap