Unravelling the reasons for disproportion in the ratio of AOB and NOB in aerobic granular sludge.

Applied Microbiology and Biotechnology
Mari K H WinklerMark C M van Loosdrecht

Abstract

In this study, we analysed the nitrifying microbial community (ammonium-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB)) within three different aerobic granular sludge treatment systems as well as within one flocculent sludge system. Granular samples were taken from one pilot plant run on municipal wastewater as well as from two lab-scale reactors. Fluorescent in situ hybridization (FISH) and quantitative PCR (qPCR) showed that Nitrobacter was the dominant NOB in acetate-fed aerobic granules. In the conventional system, both Nitrospira and Nitrobacter were present in similar amounts. Remarkably, the NOB/AOB ratio in aerobic granular sludge was elevated but not in the conventional treatment plant suggesting that the growth of Nitrobacter within aerobic granular sludge, in particular, was partly uncoupled from the lithotrophic nitrite supply from AOB. This was supported by activity measurements which showed an approximately threefold higher nitrite oxidizing capacity than ammonium oxidizing capacity. Based on these findings, two hypotheses were considered: either Nitrobacter grew mixotrophically by acetate-dependent dissimilatory nitrate reduction (ping-pong effect) or a nitrite oxidation/nitrate reduction loop (nit...Continue Reading

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Citations

Jun 19, 2015·Biotechnology and Bioengineering·T P W Vannecke, E I P Volcke
Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hanna KochHolger Daims
Jun 21, 2014·AMB Express·Helena JunickeRobbert Kleerebezem
Aug 8, 2015·Environmental Science & Technology·Mari-K H WinklerEveline I P Volcke
Jun 12, 2013·Bioresource Technology·Chunli WanXiangliang Pan
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Dec 19, 2019·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Lydia JahnJörg Krampe
Mar 10, 2020·Environmental Technology·Rodrigo de Freitas BuenoVitor Silva Liduino
Mar 14, 2020·Environmental Technology·Katarina Lydia FriedrichLuciene Alves Batista Siniscalchi
Mar 1, 2018·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Benjamin J ThwaitesRichard Stuetz
Dec 1, 2018·Water Science and Technology : a Journal of the International Association on Water Pollution Research·François-René BourgeoisDaniel G Cyr
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Sep 6, 2020·The Science of the Total Environment·Min ZhengShihu Hu
Dec 15, 2020·The Science of the Total Environment·Benjamin J ThwaitesBen van den Akker
Jun 9, 2020·Journal of Environmental Management·Sharjeel WaqasMuhammad Aslam

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Methods Mentioned

BETA
PCR

Software Mentioned

MFCS

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