Unravelling the role of the ToxR-like transcriptional regulator WmpR in the marine antifouling bacterium Pseudoalteromonas tunicata

Microbiology
Sacha StelzerStaffan Kjelleberg

Abstract

The dark-green-pigmented marine bacterium Pseudoalteromonas tunicata produces several target-specific compounds that act against a range of common fouling organisms, including bacteria, fungi, protozoa, invertebrate larvae and algal spores. The ToxR-like regulator WmpR has previously been shown to regulate expression of bioactive compounds, type IV pili and biofilm formation phenotypes which all appear at the onset of stationary phase. In this study a comparison of survival under starvation or stress between the wild-type P. tunicata strain and a wmpR mutant (D2W2) does not suggest a role for WmpR in regulating starvation- and stress-resistant phenotypes such as those that may be required in stationary phase. Both proteomic [2-dimensional PAGE (2D-PAGE)] and transcriptomic (RNA arbitrarily primed PCR) studies were used to discover members of the WmpR regulon. 2D-PAGE identified 11 proteins that were differentially expressed by WmpR. Peptide sequence data were obtained for six of these proteins and identified using the draft P. tunicata genome as being involved in protein synthesis, amino acid transamination and ubiquinone biosynthesis, as well as hypothetical proteins. The transcriptomic analysis identified three genes signific...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1987·Analytical Biochemistry·B Schwyn, J B Neilands
Aug 1, 1996·Applied and Environmental Microbiology·S G JamesS Kjelleberg
Feb 17, 1998·Applied and Environmental Microbiology·J R MarchesiW G Wade
Nov 26, 1998·International Journal of Systematic Bacteriology·C HolmströmS Kjelleberg
Apr 28, 2000·Microbiology·B Søballe, R K Poole
Oct 12, 2000·FEMS Microbiology Letters·M C Moffitt, B A Neilan
Mar 15, 2001·FEMS Microbiology Ecology·S EganS Kjelleberg
Apr 20, 2001·FEMS Microbiology Ecology·M V. Brown, J P. Bowman
May 22, 2001·Environmental Microbiology·A J HolmesM R Gillings
Jul 27, 2001·Archives of Biochemistry and Biophysics·G I BelogrudovC F Clarke
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Iain L LamontMichael L Vasil
Aug 3, 2002·Environmental Microbiology·Suhelen EganStaffan Kjelleberg
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Qing XuJohn J Mekalanos
Feb 4, 2003·Nature Reviews. Molecular Cell Biology·José D Faraldo-Gómez, Mark S P Sansom
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·James BinaJohn Mekalanos
May 7, 2003·Current Opinion in Microbiology·Eric S Krukonis, Victor J DiRita
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Jan 1, 2004·The Journal of Antibiotics·Kaneo KanohYoshikazu Shizuri
Jun 9, 2004·Applied and Environmental Microbiology·Anne Mai-ProchnowStaffan Kjelleberg
Jul 1, 2004·Molecular Microbiology·Amber GoodchildRicardo Cavicchioli
Oct 27, 2004·Clinical Science·Joan López-HellínElena García-Arumí
Nov 16, 2004·Proteomics·Angelika GörgMichael J Dunn
Dec 8, 2004·Current Opinion in Structural Biology·Gregory L Challis, James H Naismith
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Deborah T Hung, John J Mekalanos
Nov 17, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashley FranksNaresh Kumar
Jul 1, 1997·Microbiology·David R NelsonStaffan Kjelleberg

❮ Previous
Next ❯

Citations

Dec 21, 2006·Biofouling·Periasamy SaravananSeetharaman Jayachandran
Jul 27, 2010·Applied and Environmental Microbiology·Luisa R Molina-QuinteroAntonio Sanchez-Amat
Aug 31, 2013·Standards in Genomic Sciences·Michael WeissDavid Schleheck
Jun 13, 2015·Medical Microbiology and Immunology·Arbia KhemiriPascal Cosette
Mar 19, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Giordanni C DantasHenrique Ferreira
May 12, 2009·Journal of Biotechnology·Noémie Manuelle Dorval CourchesneChristopher Q Lan
Jun 6, 2008·Current Opinion in Microbiology·Suhelen EganStaffan Kjelleberg
Aug 28, 2009·Molecular Microbiology·Larissa Tetsch, Kirsten Jung
May 22, 2008·Electrophoresis·Thomas SchwederMichael Hecker
Feb 12, 2019·Communications Biology·Sophie BrameyerKirsten Jung
Jan 12, 2020·Communications Biology·Sophie BrameyerKirsten Jung

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.