Unrecognized prolonged viral replication in the pathogenesis of human RSV infection

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Bindiya BaggaJohn P DeVincenzo

Abstract

Respiratory symptoms in RSV persist long after the virus is no longer detected by culture. Current concepts of RSV pathogenesis explain this by RSV inducing a long-lasting pathogenic immune cascade. We alternatively hypothesized that prolonged unrecognized RSV replication may be responsible and studied this possibility directly in a human wild-type RSV experimental infection model. The objective of the current report was to define the duration of true human RSV replication by studying it directly in immunocompetent adults experimentally infected with a clinical strain of RSV utilizing this previously established safe and reproducible model. 35 healthy adult volunteers were inoculated with RSV-A (Memphis-37, a low11 passage clinical strain virus, manufactured from a hospitalized bronchiolitic infant) and evaluated over 12 days. Viral load by culture, parallel quantitative PCR (genomic, message) and RSV-specific IgA, were measured twice daily from serially collected nasal washes. After inoculation, 77% (27/35) of volunteers became RSV infected. As expected, culture-detectable RSV ceased abruptly by the 5-6 t h 15 infection day. However, infected volunteers demonstrated prolonged RSV presence by both genomic and message PCR. RSV-s...Continue Reading

Citations

Aug 14, 2020·Influenza and Other Respiratory Viruses·Rob Lambkin-Williams, John P DeVincenzo
Dec 18, 2020·The Journal of General Virology·Peter SimmondsHeli Harvala

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