Unreliable detection of Mycobacterium xenopi by the nonradiometric Bactec MGIT 960 culture system.

Journal of Clinical Microbiology
Claudio PiersimoniGiancarlo Gherardi

Abstract

From June 2006 to December 2007, 3,648 clinical specimens consecutively received for mycobacterial culture were investigated. Each processed sample was inoculated into Bactec MGIT 960 liquid medium and a Löwenstein-Jensen slant. Tubes that were flagged as positive by the instrument as well as those determined to be negative after 42 days of incubation were removed, visually inspected for growth, and checked for the presence of acid-fast bacilli. Three hundred sixty-nine mycobacterial strains were recovered; of the 44 Mycobacterium xenopi isolates recovered by MGIT medium, only 13 were detected by the instrument (P<0.0001). Most tubes yielding M. xenopi exhibited a peculiar pattern of growth characterized by a scant number of round, yellow-pigmented granules instead of the fine, evenly dispersed clumps usually observed for mycobacteria. It is suggested to check all individual tubes discarded by the MGIT 960 system at the end of the incubation period to prevent a significant amount of previously undetected growth from being missed.

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Citations

Apr 12, 2012·Journal of Clinical Microbiology·Jeremy A PeñaJohn A Branda
Oct 30, 2010·Emerging Infectious Diseases·Rabia A AhmedCary Shandro
Feb 14, 2015·Clinics in Chest Medicine·Jakko van Ingen
Feb 2, 2018·Clinical Microbiology Reviews·Betty A ForbesMichael L Wilson
Jun 19, 2015·Journal of Clinical Microbiology·Ramzi GhodbaneMichel Drancourt
Jun 17, 2020·Andrologia·Ayodeji Folorunsho Ajayi, Roland Eghoghosoa Akhigbe
Sep 8, 2017·African Journal of Laboratory Medicine·Sharana MahomedKoleka Mlisana

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