Unreliable measurement of basophil maximum leukotriene release with the Bühlmann CAST 2000 enzyme-linked immunosorbent assay kit

Clinical and Vaccine Immunology : CVI
S E BurasteroL Vangelista

Abstract

The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcepsilonRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

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Citations

Jun 2, 2012·Journal of Allergy·Dirk Schäfer, Steffen Maune
Aug 16, 2016·Biochimica Et Biophysica Acta·Elena FerrariThelma A Pertinhez
Nov 2, 2011·International Archives of Allergy and Immunology·Elena FerrariSamuele E Burastero

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