Unresponsiveness to human leukocytes in immunosuppressed mice by combined donor-derived human transferrin and antigens

Transplant Immunology
W PierpaoliA Bardotti

Abstract

Previous work on the facilitation of xenogeneic and allogeneic bone marrow engraftment in irradiated mice and dogs with transferrins allowed the development of a model for induction of an apparently durable state of immunological unresponsiveness or 'tolerance' in chemically immunosuppressed mice. The system is based on the simultaneous and combined administration of donor-derived cell antigens, namely human leukocytes, and specific donor-derived or plasma pool human transferrin into BALB/c or C57BL/6 mice previously treated with prednisolone and cyclophosphamide on day 0 and day 1 of the experiment. A properly timed presentation of both donor-specific or plasma pool transferrin and leukocyte antigens into the mice on day 3 and day 16 of the experiment, in the course of initial restoration of their lymphohaemopoietic tissues and cells after severe immunosuppression, results 1-3 months later, in their inability to 'recognize' human donor lymphocytes and to mount an immediate or a delayed-type immune response against human antigens. This durable state of unresponsiveness was evaluated by a complement-mediated cytotoxicity assay, with a mixed lymphocyte culture method and confirmed by the abrogation of the humoral (antibody respon...Continue Reading

References

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Citations

Dec 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·V A LesnikovH J Deeg
Jun 1, 2000·Experimental Gerontology·W PierpaoliS Arrighi
Jul 14, 2000·Journal of Hematotherapy & Stem Cell Research·M LesnikovaH J Deeg
Oct 30, 2009·Leukemia & Lymphoma·H Joachim DeegHoward M Shulman
Jan 6, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Vladimir A LesnikovH Joachim Deeg

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