Unsaturated aldehydes induce CCK secretion via TRPA1 in STC-1 cells

Molecular Nutrition & Food Research
Shingo NakajimaHiroshi Hara

Abstract

Cholecystokinin (CCK) producing cells sense luminal contents to regulate the exocrine pancreas, gastric motility, and appetite. Although long-chain fatty acids (FAs, ≥ C12) are well known to stimulate CCK secretion, the CCK-releasing activities of other aliphatic compounds, such as aldehydes (Alds) or alcohols (Alcs), have not been studied. We tested the CCK-releasing activities of various aliphatic compounds with various carbon chain lengths (C3-C13) and degrees of unsaturation in the enteroendocrine cell line STC-1. CCK released from the cell was measured using an ELISA, and intracellular calcium concentration was measured using Fura-2. Mono- and di-unsaturated Alds at 100 μM, but not saturated Alds, induced CCK secretion in STC-1 cells. Alcs and FAs failed to induce CCK secretion, regardless of carbon chain length or degree of unsaturation. Unsaturated Alds increased intracellular calcium concentration, but saturated Alds, Alcs, and FAs did not. Intracellular calcium mobilization and CCK secretion induced by unsaturated Alds was abolished in the absence of extracellular calcium. In addition, the inhibition of the transient receptor potential ankyrin 1 (TRPA1) channel suppressed unsaturated Ald-induced CCK secretion and intra...Continue Reading

References

Nov 2, 1995·Biochemical and Biophysical Research Communications·C H ChangT M Chang
Jan 1, 1997·Annual Review of Physiology·R A Liddle
Apr 15, 2000·Toxicology and Applied Pharmacology·J M HyvelinR Marthan
Dec 21, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·C FeinleM Fried
Feb 8, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ixchel M BrennanChristine Feinle-Bisset
May 19, 2006·American Journal of Physiology. Cell Physiology·Monica C ChenEnrique Rozengurt
Feb 10, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sungwon ChoiGregory W Aponte
Mar 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marco PalazzoCristiano Rumio
Mar 31, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Milena BogunovicScott E Plevy
Nov 1, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Toshiki TanakaGozoh Tsujimoto
May 1, 2008·The Journal of Biological Chemistry·Paul A GrimsrudDavid A Bernlohr
Oct 11, 2008·The Journal of Clinical Investigation·Tae-Il JeonTimothy F Osborne
Feb 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Katsura NozawaHiroyuki Ito
Jul 25, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Hitoshi DoiharaHiroyuki Ito
Aug 2, 2011·Molecular Nutrition & Food Research·Giancarlo AldiniMarina Carini
Oct 15, 2011·American Journal of Physiology. Cell Physiology·Bhavik P ShahTimothy A Gilbertson
Jan 10, 2012·Pflügers Archiv : European journal of physiology·Tomoko KomatsuMakoto Tominaga
Apr 10, 2012·Cell Metabolism·Frank ReimannFiona M Gribble
Jun 23, 2012·PloS One·Arianne L Motter, Gerard P Ahern

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Citations

Apr 16, 2016·Molecular Endocrinology·Oleg G ChepurnyColin A Leech
Nov 2, 2019·Physiological Reviews·Karel TalaveraBernd Nilius
May 26, 2018·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·M BishnoiS K Panchal
Feb 5, 2016·Chemical Senses·Nathaniel T BlairDavid E Clapham
Apr 17, 2020·Food Science and Biotechnology·Hyunnho ChoHye Young Kim

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