PMID: 7035675Jan 1, 1982Paper

Unsaturated fatty acid requirement in Escherichia coli: mechanism of palmitate-induced inhibition of growth of strain WN1

The Journal of Membrane Biology
L O IngramN L Vreeland

Abstract

The minimum requirement for unsaturated fatty acids was investigated in E. coli using a mutant impaired in the synthesis of vaccenic acid. Exogenously supplied palmitic acid was incorporated by this mutant which led to a reduction in the proportion of cellular unsaturated fatty acids. Growth was impaired as the level of saturated fatty acids approached 76% at 37 degree C and 60% at 30 degree C. The basis of this growth inhibition was investigated. Most transport systems and enzymes examined remained active in palmitate-grown cells although the specific activities of glutamate uptake and succinic dehydrogenase were depressed 50%. Fluorescent probes of membrane organization indicated that fluidity decreased with palmitate incorporation. Temperature scans with parinaric acid indicated that rigid lipid domains exist in palmitate-grown cells at their respective growth temperature. Freeze-fracture electron microscopy confirmed the presence of phase separations (particle-free areas) in palmitate-grown cells held at their growth temperature prior to quenching. The extent of this separation into particle-free and particle-enriched domains was equivalent to that induced by a shift to 0 degree C in control cells. The incorporation of palm...Continue Reading

References

Jun 1, 1977·Canadian Journal of Microbiology·L O Ingram
Jan 1, 1978·Annual Review of Biochemistry·J E Cronan
Oct 4, 1978·Biochimica Et Biophysica Acta·M B Jackson, J E Cronan
Oct 19, 1979·Biochimica Et Biophysica Acta·S Marcelja, J Wolfe
May 1, 1975·European Journal of Biochemistry·M KitoS Sawada
Feb 1, 1976·Journal of Bacteriology·L O Ingram
Jan 1, 1974·Annual Review of Biochemistry·A J Fulco
May 1, 1969·Journal of Bacteriology·U HenningG Deppe
Jun 1, 1980·Journal of Bacteriology·B BergerL O Ingram
Jan 1, 1980·CRC Critical Reviews in Biochemistry·P J Quinn, D Chapman
Nov 1, 1980·Journal of Bacteriology·L O Ingram, N S Vreeland
Dec 1, 1962·Journal of Bacteriology·A G Marr, J L Ingraham
Nov 1, 1943·Genetics·S E Luria, M Delbrück

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Citations

Jul 9, 1999·Progress in Lipid Research·C C DiRussoJ D Weimar
Oct 1, 1984·The Journal of Clinical Investigation·J D CoonrodL C Hsu
Jun 1, 1983·Journal of Bacteriology·V C Carey, L O Ingram
Apr 30, 2020·Archives of Medical Research·Undurti N Das

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