PMID: 3748050Sep 1, 1986Paper

Unscheduled DNA synthesis in rat tracheal epithelial cells, hepatocytes and spermatocytes following exposure to methyl chloride in vitro and in vivo

Mutation Research
P K WorkingB E Butterworth

Abstract

Measurement of DNA repair as unscheduled DNA synthesis (UDS) in vitro following exposure in vivo in multiple tissues from the same treated animal can provide valuable information relating to the tissue- and organ-specificity of chemically induced DNA damage. UDS was evaluated in primary cultures of rat tracheal epithelial cells, hepatocytes and pachytene spermatocytes after exposure in vitro to methyl chloride (MeCl), and after isolation from the same treated animal following inhalation exposure in vivo. Concentrations of 1-10% MeCl in vitro induced UDS in hepatocytes and spermatocytes, but not in tracheal epithelial cells. Inhalation exposure to MeCl in vivo (3000-3500 ppm 6 h/day for 5 successive days) failed to induce DNA repair in any cell type. In vivo exposure to 15 000 ppm MeCl for 3 h also failed to induce UDS in tracheal epithelial cells and spermatocytes, but did cause a marginal increase in UDS in hepatocytes. Thus, MeCl appears to be a weak, direct-acting genotoxicant. While activity could be measured in hepatocytes and spermatocytes directly in vitro, only extremely high concentrations of MeCl elicited a response in the whole animal, and then only in hepatocytes.

References

Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G J ChellmanP K Working
Aug 1, 1982·Toxicology and Applied Pharmacology·D J KornbrustJ A Swenberg
May 1, 1981·Science·S A Weitzman, T P Stossel
Nov 1, 1984·Toxicology and Applied Pharmacology·R E ChapinJ S Bus
Nov 1, 1984·Carcinogenesis·E Bermudez, P F Allen
Jun 1, 1984·Carcinogenesis·D J Doolittle, B E Butterworth
Jan 1, 1984·Environmental Mutagenesis·P K Working, B E Butterworth
Jan 1, 1982·Environmental Mutagenesis·J C MirsalisB E Butterworth
Nov 20, 1952·Annals of the New York Academy of Sciences·C P LEBLOND, Y CLERMONT

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Citations

Dec 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S Y Brendler-SchwaabB L Pool-Zobel
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G J ChellmanP K Working
Oct 1, 1986·Toxicology and Applied Pharmacology·G J ChellmanJ S Bus
Mar 1, 1997·Toxicology and Applied Pharmacology·S Cantoreggi, D A Keller
Jan 1, 1993·Critical Reviews in Toxicology·H M Bolt, B Gansewendt

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