Unspinning chromatin: Revealing the dynamic nucleosome landscape by NMR

Progress in Nuclear Magnetic Resonance Spectroscopy
Clara L van Emmerik, Hugo van Ingen

Abstract

NMR is an essential technique for obtaining information at atomic resolution on the structure, motions and interactions of biomolecules. Here, we review the contribution of NMR to our understanding of the fundamental unit of chromatin: the nucleosome. Nucleosomes compact the genome by wrapping the DNA around a protein core, the histone octamer, thereby protecting genomic integrity. Crucially, the imposed barrier also allows strict regulation of gene expression, DNA replication and DNA repair processes through an intricate system of histone and DNA modifications and a wide range of interactions between nucleosomes and chromatin factors. In this review, we describe how NMR has contributed to deciphering the molecular basis of nucleosome function. Starting from pioneering studies in the 1960s using natural abundance NMR studies, we focus on the progress in sample preparation and NMR methodology that has allowed high-resolution studies on the nucleosome and its subunits. We summarize the results and approaches of state-of-the-art NMR studies on nucleosomal DNA, histone complexes, nucleosomes and nucleosomal arrays. These studies highlight the particular strength of NMR in studying nucleosome dynamics and nucleosome-protein interact...Continue Reading

Citations

Jun 23, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Rajivgandhi Sundaram, Dileep Vasudevan
Mar 12, 2020·Frontiers in Molecular Biosciences·Artemi BendandiWalter Rocchia
May 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Gili AbramovLewis E Kay
Jun 14, 2020·International Journal of Molecular Sciences·Balázs Zoltán Zsidó, Csaba Hetényi
Jun 13, 2020·Mechanisms of Ageing and Development·Samir Kumar Patra
Dec 15, 2020·Journal of Molecular Biology·Jan Huertas, Vlad Cojocaru
Feb 20, 2021·IScience·Catherine A Musselman, Tatiana G Kutateladze
Oct 26, 2020·Journal of Molecular Biology·Yunhui PengAnna R Panchenko
Apr 21, 2020·Journal of Structural Biology·Romain RetureauBrigitte Hartmann

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