PMID: 8603105Mar 27, 1996Paper

Unstimulated platelets evoke calcium responses in human umbilical vein endothelial cells

Biochimica Et Biophysica Acta
S C van IJzendoornJ W Heemskerk

Abstract

Interactions between human platelets and human umbilical vein endothelial cells (HUVEC) were studied by monitoring changes in cytosolic [Ca2+]i in both cell types. Confluent monolayers of Fura-2-loaded HUVEC, grown on gelatin-coated coverslips, responded to repeated addition of a suspension of unstimulated platelets by transient increases in cytosolic [Ca2+]i. These platelet-evoked Ca2+ responses were not caused by products secreted from the platelets and were insensitive to inhibitors of platelet activation and/or platelet aggregation. The platelet-evoked rises in [Ca2+]i in endothelial cells, similarly as the thrombin-evoked rises, were blocked by preincubation of HUVEC with the phospholipase C inhibitor U73122 or the Ca2+ influx blocker Ni2+. In contrast, treatment with the protein tyrosine kinase inhibitor genistein was without effect. Video imaging experiments, in which the fluorescence signal was analysed from the individual cells of an endothelial monolayer, showed that only 2-20% of the cells, scattered over the monolayer, responded to the addition of platelets by a transient increase in [Ca2+]i, whereas most of the cells responded to thrombin. This leads to the conclusion that unstimulated platelets can activate HUVEC ...Continue Reading

References

Feb 11, 1992·The American Journal of Physiology·J ShenM A Gimbrone
Jan 30, 1990·Biochemical and Biophysical Research Communications·R C AshmoreK M VanBenthuysen
Jul 16, 1990·Biochemical and Biophysical Research Communications·M U NollertL V McIntire
Jan 1, 1989·Physiological Reviews·W Siess
Jul 25, 1994·FEBS Letters·C P ReutelingspergerA Tiebosch
Mar 1, 1994·The American Journal of Physiology·M J Kuchan, J A Frangos

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Citations

Aug 27, 2005·American Journal of Respiratory Cell and Molecular Biology·Maimaiti T YimingSunita Bhattacharya
Dec 24, 1998·British Journal of Pharmacology·E Pipili-SynetosM E Maragoudakis
Oct 29, 2000·Archives of Biochemistry and Biophysics·R M van GorpJ W Heemskerk

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