Unstructured RNA is a substrate for tRNase Z

Biochemistry
Hirotaka S ShibataM Nashimoto

Abstract

tRNase Z, which exists in almost all cells, is believed to be working primarily for tRNA 3' maturation. In Escherichia coli, however, the tRNase Z gene appears to be dispensable under normal growth conditions, and its physiological role is not clear. Here, to investigate a possibility that E. coli tRNase Z cleaves RNAs other than pre-tRNAs, we tested several unstructured RNAs for cleavage. Surprisingly, all these substrates were cleaved very efficiently at multiple sites by a recombinant E. coli enzyme in vitro. tRNase Zs from Bacillus subtilis and Thermotoga maritima also cleaved various unstructured RNAs. The E. coli and B. subtilis enzymes seem to have a tendency to cleave after cytidine or before uridine, while cleavage by the T. maritima enzyme inevitably occurred after CCA in addition to the other cleavages. Assays to determine optimal conditions indicated that metal ion requirements differ between B. subtilis and T. maritima tRNase Zs. There was no significant difference in the observed rate constant between unstructured RNA and pre-tRNA substrates, while the K(d) value of a tRNase Z/unstructured RNA complex was much higher than that of an enzyme/pre-tRNA complex. Furthermore, eukaryotic tRNase Zs from yeast, pig, and hu...Continue Reading

References

Mar 17, 2001·EMBO Reports·M Mörl, A Marchfelder
Feb 6, 2003·International Journal of Cancer. Journal International Du Cancer·Wouter KorverEmma Lees
Jan 30, 2004·The Journal of Biological Chemistry·Asako MinagawaMasayuki Nashimoto
Feb 9, 2005·The Journal of Biological Chemistry·Oliver SchillingAndreas Vogel
Mar 15, 2005·The Journal of Biological Chemistry·Benjamin EzratyMurray P Deutscher
Apr 13, 2005·The Journal of Biological Chemistry·Hirotaka S ShibataMasayuki Nashimoto
Aug 25, 2005·The Journal of Biological Chemistry·Bettina SpäthAnita Marchfelder

❮ Previous
Next ❯

Citations

Mar 17, 2007·Nature Reviews. Microbiology·Yulia RedkoCiarán Condon
Apr 16, 2009·The Journal of Biological Chemistry·Tanmay Dutta, Murray P Deutscher
Apr 16, 2009·The Journal of Biological Chemistry·Kateryna PodzelinskaZongchao Jia
Sep 3, 2010·Genes & Development·Eric M Phizicky, Anita K Hopper
Jun 25, 2008·FEBS Letters·Masayuki TakahashiMasayuki Nashimoto
Apr 25, 2007·Critical Reviews in Biochemistry and Molecular Biology·Zbigniew Dominski
Oct 1, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroaki Takaku, Masayuki Nashimoto
Jul 28, 2010·FEMS Microbiology Reviews·Cecília M ArraianoSandra C Viegas
Jul 8, 2008·Journal of Molecular Biology·Asako MinagawaMasayuki Nashimoto
Sep 1, 2018·Bioinformatics·Chun-Chi ChenByung-Jun Yoon
Oct 31, 2020·PloS One·Yoshiki KatoAkiko Shimizu-Ibuka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.