Untargeted and Targeted Metabolomics and Tryptophan Decarboxylase In Vivo Characterization Provide Novel Insight on the Development of Kiwifruits (Actinidia deliciosa )

International Journal of Molecular Sciences
Mauro CommissoFlavia Guzzo

Abstract

Kiwifruit (Actinidia deliciosa cv. Hayward) is a commercially important crop with highly nutritional green fleshy fruits. The post-harvest maturation of the fruits is well characterized, but little is known about the metabolic changes that occur during fruit development. Here we used untargeted metabolomics to characterize the non-volatile metabolite profile of kiwifruits collected at different time points after anthesis, revealing profound metabolic changes before the onset of ripening including the depletion of many classes of phenolic compounds. In contrast, the phytohormone abscisic acid accumulated during development and ripening, along with two indolamines (serotonin and its precursor tryptamine), and these were monitored in greater detail by targeted metabolomics. The role of indolamines in kiwifruit development is completely unknown, so we also characterized the identity of genes encoding tryptophan decarboxylase in A. deliciosa and its close relative A. chinensis to provide insight into the corresponding biological processes. Our results indicate that abscisic acid and indolamines fulfill unrecognized functions in the development and ripening of kiwifruits.

References

Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·V De LucaN Brisson
May 4, 2000·Journal of Agricultural and Food Chemistry·H M Dawes, J B Keene
Jun 21, 2002·Asia Pacific Journal of Clinical Nutrition·Elaine C RushLynnette R Ferguson
Sep 5, 2002·Journal of Agricultural and Food Chemistry·María J JordánKevin L Goodner
Oct 1, 1995·Plant Physiology·J. C. ThomasH. J. Bohnert
Oct 1, 1993·The Plant Cell·G. GillaspyW. Gruissem
Oct 3, 2002·Journal of Agricultural and Food Chemistry·Mendel Friedman
Jun 1, 1960·The Journal of Pharmacy and Pharmacology·J M FOY, J R PARRATT
Dec 1, 1959·Archives of Biochemistry and Biophysics·S UDENFRIENDA SJOERDSMA
Jul 20, 2006·International Journal of Food Sciences and Nutrition·Yong-Seo ParkShela Gorinstein
Aug 31, 2007·World Journal of Gastroenterology : WJG·Annie-On-On ChanNina-Yh Wong
Nov 9, 2007·The Plant Journal : for Cell and Molecular Biology·Navot GalpazJoseph Hirschberg
May 23, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Angélique StalmachAlan Crozier
Feb 2, 2010·International Journal of Food Sciences and Nutrition·Piotr LatochaJacek Wilczak
May 28, 2011·Plant Signaling & Behavior·Akula RamakrishnaGokare Aswathanarayana Ravishankar
Jul 23, 2011·Journal of Experimental Botany·Ye-Mao ChaiYuan-Yue Shen
Dec 30, 2011·BMC Plant Biology·Annette C RichardsonRobert J Schaffer
Mar 20, 2012·Journal of Plant Physiology·L NitschI Rieu
Oct 15, 2013·Journal of Experimental Botany·Alistair J HallMichel Génard
Oct 19, 2013·Journal of Agricultural and Food Chemistry·Renato BoscoValentina Scariot
Oct 19, 2013·Nature Communications·Shengxiong HuangYongsheng Liu
Jan 18, 2014·Journal of Pineal Research·Yeong ByeonKyoungwhan Back
Jan 22, 2014·Journal of Experimental Botany·Takayuki TohgeAlisdair R Fernie
May 14, 2014·Journal of Experimental Botany·Ping LengYangdong Guo
Jul 21, 2014·Food Chemistry·Yong-Seo ParkShela Gorinstein

❮ Previous
Next ❯

Citations

Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Martino BianconiFlavia Guzzo
Dec 29, 2019·Food Research International·Murilo DiasIsabel C F R Ferreira
May 20, 2021·Journal of Experimental Botany·Stefano NegriFlavia Guzzo

❮ Previous
Next ❯

Datasets Mentioned

BETA
MK442011
ACN62127.1
AAA33109.1

Methods Mentioned

BETA
PCA
X-ray
PCR

Software Mentioned

Clustal Omega
Xevo
MassLynx
TargetP
BlastP
MegaX
LinRegPCR
Data Analysis
ClustalW
Progenesis QI

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.