Untargeted LC-MS Metabolomics Differentiates Between Virulent and Avirulent Clinical Strains of Pseudomonas aeruginosa.

Biomolecules
Tobias DepkeMark Brönstrup

Abstract

Pseudomonas aeruginosa is a facultative pathogen that can cause, inter alia, acute or chronic pneumonia in predisposed individuals. The gram-negative bacterium displays considerable genomic and phenotypic diversity that is also shaped by small molecule secondary metabolites. The discrimination of virulence phenotypes is highly relevant to the diagnosis and prognosis of P. aeruginosa infections. In order to discover small molecule metabolites that distinguish different virulence phenotypes of P. aeruginosa, 35 clinical strains were cultivated under standard conditions, characterized in terms of virulence and biofilm phenotype, and their metabolomes were investigated by untargeted liquid chromatography-mass spectrometry. The data was both mined for individual candidate markers as well as used to construct statistical models to infer the virulence phenotype from metabolomics data. We found that clinical strains that differed in their virulence and biofilm phenotype also had pronounced divergence in their metabolomes, as underlined by 332 features that were significantly differentially abundant with fold changes greater than 1.5 in both directions. Important virulence-associated secondary metabolites like rhamnolipids, alkyl quinol...Continue Reading

References

Jun 1, 1995·Epidemiology and Infection·F StapletonM Matheson
Apr 6, 2002·American Journal of Respiratory and Critical Care Medicine·Jean Chastre, Jean-Yves Fagon
Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Matthew C WolfgangStephen Lory
Jul 21, 2004·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Karin SchlegelHerbert Budzikiewicz
Aug 13, 2005·Bioinformatics·Tobias SingThomas Lengauer
Aug 17, 2005·Molecular Microbiology·Blaise R BolesPradeep K Singh
Nov 23, 2005·Archives of Otolaryngology--head & Neck Surgery·Eric W WangRichard A Chole
May 25, 2006·Annual Review of Phytopathology·Dmitri V MavrodiLinda S Thomashow
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kalai MatheeStephen Lory
Mar 23, 2010·Journal of Bacteriology·Gregory C PalmerMarvin Whiteley
May 1, 2010·Journal of Medical Microbiology·R S BradburyA C Champion
Jun 18, 2010·Environmental Microbiology·Eliane FrimmersdorfDietmar Schomburg
Oct 29, 2010·Genome Research·Gerton Lunter, Martin Goodson
Dec 15, 2010·PLoS Neglected Tropical Diseases·Ruben t'KindtSaskia Decuypere
Apr 1, 2011·Environmental Microbiology·Sophie de Bentzmann, Patrick Plésiat
Mar 7, 2012·Microbiology and Molecular Biology Reviews : MMBR·Pol Nadal JimenezWim J Quax
Mar 23, 2012·Nature Reviews. Molecular Cell Biology·Gary J PattiGary Siuzdak
Apr 27, 2012·Analytical Chemistry·Ralf TautenhahnGary Siuzdak
Apr 11, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Tianlu ChenAihua Zhao
Dec 3, 2013·Frontiers in Cellular and Infection Microbiology·Pierre Cornelis, Jozef Dingemans
Mar 5, 2014·F1000prime Reports·Burkhard TümmlerNina Cramer
Apr 30, 2014·BMC Genomics·María-Victoria Grosso-BecerraGloria Soberón-Chávez
Aug 27, 2014·Environmental Microbiology·Rolf HilkerBurkhard Tümmler
Aug 28, 2014·Analytical Chemistry·Nathaniel Guy MahieuGary J Patti
Sep 14, 2014·Analytical and Bioanalytical Chemistry·Peng Gao, Guowang Xu
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Dec 2, 2015·The ISME Journal·Robert A QuinnForest L Rohwer
Mar 12, 2016·Analytica Chimica Acta·Lunzhao YiYizeng Liang
May 14, 2016·Expert Review of Respiratory Medicine·Helena Lund-PalauJane C Davies
Nov 22, 2016·Journal of Breath Research·Heather D BeanJane E Hill
Jan 18, 2017·Bioorganic & Medicinal Chemistry·Nikolaus GuttenbergerRolf Breinbauer
Jun 24, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tobias DepkeMark Brönstrup
Aug 11, 2017·F1000Research·Jens Klockgether, Burkhard Tümmler
Apr 6, 2018·Nature Biotechnology·Carlos GuijasGary Siuzdak

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
PCA

Software Mentioned

CAMERA
Mass Spectrometry Search Tool ( MASST )
tidyverse
DESeq2
vegan R package
ROCR ’ R
MetaboLights
XCMSonline
R package DESeq2
MASST

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.