Untargeted lipidomic analysis of human tears: A new approach for quantification of O-acyl-omega hydroxy fatty acids.

The Ocular Surface
Jianzhong ChenJason J Nichols

Abstract

The purpose of this work was to optimize methodology to analyze the human tear film lipids by using untargeted, direct infusion electrospray ionization-mass spectrometry (ESI-MS) to establish the analytical approach for a large-scale clinical translational study of tear film lipids in ocular surface disease, particularly associated with the O-acyl-omega hydroxy fatty acids (OAHFAs). Meibum and tear samples were collected from both eyes of five normal subjects without ocular disease using two different microcapillary collection tubes, glass and polytetrafluoroethylene (PTFE). An untargeted lipidomics approach was used to analyze the lipids in human tear and meibum samples using direct infusion ESI-MS in positive and negative ion modes. Direct and indirect quantification methods were evaluated. The amount of OAHFAs measured in tears using these techniques was approximately 0.7-0.8% of the total lipids. More phospholipids, including phosphatidylcholine and sphingomyelin, were detected in the tear samples associated with glass microcapillaries compared to PTFE. Reliable assessment of lipids in small volumes of tear film is possible using high resolution, untargeted ESI-MS in positive and negative ion modes. Using this technique, th...Continue Reading

Citations

Jun 20, 2020·Journal of Lipid Research·Douglas Borchman
Feb 9, 2021·Journal of Lipid Research·Douglas Borchman
Mar 25, 2021·The Ocular Surface·Jason J NicholsNathan Efron
Mar 28, 2021·The Ocular Surface·Yuqiang BaiJason J Nichols
May 22, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Romain MagnyNicolas Auzeil
Aug 28, 2021·International Journal of Molecular Sciences·Fredrik FineideHilde Kanli Galtung
Jul 2, 2019·The Journal of Physical Chemistry Letters·Riku O PaananenIlpo Vattulainen
Dec 31, 2021·Biophysical Journal·Xiaojie XuYi Y Zuo
Jan 31, 2022·Survey of Ophthalmology·Raoul K KhannaPierre-Jean Pisella

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