Untargeted Lipidomics Reveals Differences in the Lipid Pattern among Clinical Isolates of Staphylococcus aureus Resistant and Sensitive to Antibiotics

Journal of Proteome Research
Weronika Hewelt-BelkaAgata Kot-Wasik

Abstract

Staphylococcus aureus resistance to antibiotics is a significant clinical problem worldwide. In this study, an untargeted lipidomics approach was used to compare the lipid fingerprints of S. aureus clinical isolates that are resistant and sensitive to antibiotics. High-performance liquid chromatography coupled with time-of-flight mass spectrometry was employed to rapidly and comprehensively analyze bacterial lipids. Chemometric and statistical analyses of the obtained lipid fingerprints revealed variations in several lipid groups between S. aureus strains resistant and sensitive to tested antibiotics including methicillin, gentamicin, ciprofloxacin, erythromycin, and fusidic acid. The levels of identified monoglycosyldiacylglycerol, phosphatidylglycerol, and diglycosyldiacylglycerol lipid groups were found to be upregulated in antibiotic-resistant S. aureus strains, whereas the levels of diacylglycerol lipid groups were downregulated. Differences in the lipid patterns between sensitive and resistant S. aureus strains suggest that antibiotic susceptibility may be associated with the lipid composition of bacterial cells. The lipids that were found to significantly differ between antibiotic-resistant and antibiotic-sensitive clini...Continue Reading

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Citations

Aug 11, 2016·Biochemistry·Samuel Furse, David J Scott
Mar 28, 2018·Journal of Bacteriology·Phillip C DelektaNeal D Hammer
Jan 25, 2018·Scientific Reports·Eric Hong Jian WongMun Fai Loke
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Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Ann-Britt Schäfer, Michaela Wenzel
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Urszula Wójcik-BojekBeata Sadowska
May 21, 2021·Journal of the American Society for Mass Spectrometry·Christian FreemanKelly M Hines
Sep 21, 2021·Frontiers in Veterinary Science·Laerte Marlon SantosRicardo Wagner Portela

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