Untargeted Metabolomic Analysis of Capsicum spp. by GC-MS

Phytochemical Analysis : PCA
Bianca Camargo AranhaFabio Clasen Chaves

Abstract

In order to conserve the biodiversity of Capsicum species and find genotypes with potential to be utilised commercially, Embrapa Clima Temperado maintains an active germplasm collection (AGC) that requires characterisation, enabling genotype selection and support for breeding programmes. The objective of this study was to characterise pepper accessions from the Embrapa Clima Temperado AGC and differentiate species based on their metabolic profile using an untargeted metabolomics approach. Cold (-20°C) methanol extraction residue of freeze-dried fruit samples was partitioned into water/methanol (A) and chloroform (B) fractions. The polar fraction (A) was derivatised and both fractions (A and B) were analysed by gas chromatography coupled to mass spectrometry (GC-MS). Data from each fraction was analysed using a multivariate principal component analysis (PCA) with XCMS software. Amino acids, sugars, organic acids, capsaicinoids, and hydrocarbons were identified. Outlying accessions including P116 (C. chinense), P46, and P76 (C. annuum) were observed in a PCA plot mainly due to their high sucrose and fructose contents. PCA also indicated a separation of P221 (C. annuum) and P200 (C. chinense), because of their high dihydrocapsaici...Continue Reading

References

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Dec 18, 2016·Journal of Agricultural and Food Chemistry·Jessica Fernanda HoffmannFabio Clasen Chaves

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Citations

Apr 4, 2019·Plant Cell Reports·Magda Lisette Arce-Rodríguez, Neftalí Ochoa-Alejo
Jul 2, 2021·Horticulture Research·Tyler J MasonJessica E Prenni
Nov 26, 2020·Food Research International·Maria Inês DielJoão Alberto Zemolin

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