Untargeted metabolomic analysis of tomato pollen development and heat stress response

Plant Reproduction
Marine Josephine PaupièreArnaud Bovy

Abstract

Pollen development metabolomics. Developing pollen is among the plant structures most sensitive to high temperatures, and a decrease in pollen viability is often associated with an alteration of metabolite content. Most of the metabolic studies of pollen have focused on a specific group of compounds, which limits the identification of physiologically important metabolites. To get a better insight into pollen development and the pollen heat stress response, we used a liquid chromatography-mass spectrometry platform to detect secondary metabolites in pollen of tomato (Solanum lycopersicum L.) at three developmental stages under control conditions and after a short heat stress at 38 °C. Under control conditions, the young microspores accumulated a large amount of alkaloids and polyamines, whereas the mature pollen strongly accumulated flavonoids. The heat stress treatment led to accumulation of flavonoids in the microspore. The biological role of the detected metabolites is discussed. This study provides the first untargeted metabolomic analysis of developing pollen under a changing environment that can serve as reference for further studies.

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Citations

Jul 2, 2019·The New Phytologist·Monica BorghiAlisdair R Fernie
May 12, 2019·Plant, Cell & Environment·James P Santiago, Thomas D Sharkey
Jun 20, 2019·International Journal of Molecular Sciences·Xianlong DingShouping Yang
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Marine J PaupièreArnaud G Bovy
Dec 19, 2019·Metabolites·Ali RazzaqFozia Saleem
Aug 17, 2019·Frontiers in Plant Science·Yonghua LiuYouhong Song
Sep 29, 2019·Journal of Experimental Botany·Neeta LohaniPrem L Bhalla
Jan 26, 2021·Plant Science : an International Journal of Experimental Plant Biology·Aitor VillateOier Aizpurua-Olaizola
Apr 6, 2021·The New Phytologist·Palak ChaturvediDavid Honys
Jun 3, 2021·Antibiotics·Nikos Asoutis DidarasDimitris Mossialos
Jun 3, 2021·Insects·Charlotte DescampsAnne-Laure Jacquemart
Jun 22, 2021·Frontiers in Plant Science·Dandan YangGang Lu
Jul 8, 2021·Plant Reproduction·Maurizio Iovane, Giovanna Aronne

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Methods Mentioned

BETA
fluorescence microscopy
NMR
PCA
acylation
environmental stresses
glycosylation

Software Mentioned

SPSS
GeneMaths XT
GenStat
MetAlign
MSClust
Excalibur

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