Untranslated regions of diverse plant viral RNAs vary greatly in translation enhancement efficiency.

BMC Biotechnology
Qiuling FanW Allen Miller

Abstract

Whole plants or plant cell cultures can serve as low cost bioreactors to produce massive amounts of a specific protein for pharmacological or industrial use. To maximize protein expression, translation of mRNA must be optimized. Many plant viral RNAs harbor extremely efficient translation enhancers. However, few of these different translation elements have been compared side-by-side. Thus, it is unclear which are the most efficient translation enhancers. Here, we compare the effects of untranslated regions (UTRs) containing translation elements from six plant viruses on translation in wheat germ extract and in monocotyledenous and dicotyledenous plant cells. The highest expressing uncapped mRNAs contained viral UTRs harboring Barley yellow dwarf virus (BYDV)-like cap-independent translation elements (BTEs). The BYDV BTE conferred the most efficient translation of a luciferase reporter in wheat germ extract and oat protoplasts, while uncapped mRNA containing the BTE from Tobacco necrosis virus-D translated most efficiently in tobacco cells. Capped mRNA containing the Tobacco mosaic virus omega sequence was the most efficient mRNA in tobacco cells. UTRs from Satellite tobacco necrosis virus, Tomato bushy stunt virus, and Crucifer...Continue Reading

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Citations

Aug 6, 2014·Archives of Virology·Muhammad AliMuhammad Tahir
Nov 13, 2012·BMC Biotechnology·Shaveta Kanoria, Pradeep Kumar Burma
Feb 24, 2016·Molecular Plant-microbe Interactions : MPMI·W Allen MillerPulkit Kanodia
Apr 11, 2018·Plant Biotechnology Journal·Andrew G Diamos, Hugh S Mason
Apr 22, 2017·Frontiers in Plant Science·Manuel MirasMiguel A Aranda
May 21, 2020·Frontiers in Bioengineering and Biotechnology·Benjamin Bruno GengenbachJohannes Felix Buyel
Apr 13, 2021·Frontiers in Molecular Biosciences·Marie-Laure FogeronAnja Böckmann

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Datasets Mentioned

BETA
X07653
M21958
Z29370

Methods Mentioned

BETA
transgenic
electrophoresis
PCR
reverse transcription PCR

Software Mentioned

GraphPad Prism

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