Untreated Chlorella homosphaera biomass allows for high rates of cell wall glucan enzymatic hydrolysis when using exoglucanase-free cellulases

Biotechnology for Biofuels
Marcoaurélio Almenara RodriguesElba Pinto da Silva Bon

Abstract

Chlorophyte microalgae have a cell wall containing a large quantity of cellulose Iα with a triclinic unit cell hydrogen-bonding pattern that is more susceptible to hydrolysis than that of the cellulose Iβ polymorphic form that is predominant in higher plants. This study addressed the enzymatic hydrolysis of untreated Chlorella homosphaera biomass using selected enzyme preparations, aiming to identify the relevant activity profile for the microalgae cellulose hydrolysis. Enzymes from Acremonium cellulolyticus, which secretes a complete pool of cellulases plus β-glucosidase; Trichoderma reesei, which secretes a complete pool of cellulases with low β-glucosidase; Aspergillus awamori, which secretes endoglucanases and β-glucosidase; blends of T. reesei-A. awamori or A. awamori-A. cellulolyticus enzymes; and a purified A. awamori β-glucosidase were evaluated. The highest initial glucan hydrolysis rate of 140.3 mg/g/h was observed for A. awamori enzymes with high β-glucosidase, low endoglucanase, and negligible cellobiohydrolase activities. The initial hydrolysis rates when using A. cellulolyticus or T. reesei enzymes were significantly lower, whereas the results for the T. reesei-A. awamori and A. awamori-A. cellulolyticus blends we...Continue Reading

References

Oct 12, 2000·Enzyme and Microbial Technology·A M IllmanS W Shales
Dec 13, 2002·Eukaryotic Cell·Alison W RobertsDeborah P Delmer
Oct 16, 1952·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·E NICOLAI, R D PRESTON
Nov 1, 1958·The Biochemical Journal·D H NORTHCOTER W HORNE
Aug 6, 2004·Tree Physiology·Pak S Chow, Simon M Landhäusser
Dec 1, 1994·Applied and Environmental Microbiology·K S KwonY C Hah
Apr 20, 2006·Letters in Applied Microbiology·K WatanabeH Tanaka
Jan 20, 1984·Science·R H Atalla, D L Vanderhart
Dec 10, 2009·Journal of Microbiology and Biotechnology·Thuy Vu An NguyenSang Yup Lee
Mar 12, 2010·Bioresource Technology·Seung Phill ChoiSang Jun Sim
Jun 29, 2010·Bioresource Technology·Jorge Alberto Vieira Costa, Michele Greque de Morais
Jul 3, 2010·Bioresource Technology·Jon K PittmanOlumayowa Osundeko
Jul 29, 2010·Bioresource Technology·Rojan P JohnAshok Pandey
Jun 4, 2011·Enzyme Research·Marcoaurélio Almenara Rodrigues, Elba Pinto da Silva Bon
Nov 19, 2011·Bioresource Technology·J R MirandaL Gouveia
Jan 5, 2012·Enzyme Research·Sulaiman Al-ZuhairLucia Gardossi
Mar 1, 2012·Bioresource Technology·Joshua T EllisCharles D Miller
Nov 3, 2012·Bioresource Technology·Shih-Hsin HoJo-Shu Chang
Mar 1, 1984·Planta·E Loos, D Meindl
Dec 3, 2013·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Leda Maria Fortes GottschalkElba Pinto da Silva Bon

❮ Previous
Next ❯

Citations

Oct 20, 2015·Applied Microbiology and Biotechnology·Thamy Lívia Ribeiro CorrêaGonçalo Amarante Guimarães Pereira
Jul 28, 2020·Bioresource Technology·Sharlene Silva CostaLucielen Oliveira Santos

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
scanning electron microscopy
light scattering
scraping
gel filtration
gel
ion-exchange chromatography

Software Mentioned

MINITAB
SigmaPlot
Avicel Fluka

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.