Unusual ADP-forming acetyl-coenzyme A synthetases from the mesophilic halophilic euryarchaeon Haloarcula marismortui and from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum

Archives of Microbiology
Christopher Bräsen, Peter Schonheit

Abstract

ADP-forming acetyl-CoA synthetase (ACD), the novel enzyme of acetate formation and energy conservation in archaea Acety - CoA + ADP + Pi<==>acetate + ATP CoA), has been studied only in few hyperthermophilic euryarchaea. Here, we report the characterization of two ACDs with unique molecular and catalytic features, from the mesophilic euryarchaeon Haloarcula marismortui and from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum. ACD from H. marismortui was purified and characterized as a salt-dependent, mesophilic ACD of homodimeric structure (166 kDa). The encoding gene was identified in the partially sequenced genome of H. marismortui and functionally expressed in Escherichia coli. The recombinant enzyme was reactivated from inclusion bodies following solubilization and refolding in the presence of salts. The ACD catalyzed the reversible ADP- and Pi-dependent conversion of acetyl-CoA to acetate. In addition to acetate, propionate, butyrate, and branched-chain acids (isobutyrate, isovalerate) were accepted as substrates, rather than the aromatic acids, phenylacetate and indol-3-acetate. In the genome of P. aerophilum, the ORFs PAE3250 and PAE 3249, which code for alpha and beta subunits of an ACD, overlap each other by 1...Continue Reading

References

Sep 1, 1993·Applied and Environmental Microbiology·P VölklK O Stetter
Jun 21, 2001·Archives of Microbiology·C Bräsen, P Schönheit
Apr 1, 1990·International Journal of Systematic Bacteriology·A OrenB E Volcani
Jan 1, 1995·World Journal of Microbiology & Biotechnology·P Schönheit, T Schäfer

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Citations

Aug 28, 2013·Nature Communications·Cindy J CastelleJillian F Banfield
Jan 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Renato H-J WeißeAxel J Scheidig
Feb 10, 2021·Journal of Bacteriology·Tom KupratPeter Schönheit
Nov 1, 2005·Current Opinion in Microbiology·Bettina Siebers, Peter Schönheit

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